Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28831 | 3' | -56.2 | NC_006146.1 | + | 96703 | 0.67 | 0.848221 |
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Target: 5'- aCACCGGUGUGuGGUUaGCCa-CACCAUAu -3' miRNA: 3'- -GUGGUCGCAC-UCGA-CGGaaGUGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 152541 | 0.66 | 0.878541 |
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Target: 5'- gACCAGgGUGAGCaUGCC---ACCAa- -3' miRNA: 3'- gUGGUCgCACUCG-ACGGaagUGGUgu -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 160203 | 0.66 | 0.885581 |
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Target: 5'- uCGCCAGUcUGuGCUGCCUgCAugaugcCCGCGg -3' miRNA: 3'- -GUGGUCGcACuCGACGGAaGU------GGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 69933 | 0.66 | 0.892396 |
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Target: 5'- gACCAGgGUGAGCcGCCccgCGCUg-- -3' miRNA: 3'- gUGGUCgCACUCGaCGGaa-GUGGugu -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 41323 | 0.66 | 0.892396 |
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Target: 5'- -uUCAGaggGAGCUGucCCUUCACCACc -3' miRNA: 3'- guGGUCgcaCUCGAC--GGAAGUGGUGu -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 58851 | 0.66 | 0.89898 |
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Target: 5'- aACCAcCGcUGAGCUGCCcacccCACCugAc -3' miRNA: 3'- gUGGUcGC-ACUCGACGGaa---GUGGugU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 115365 | 0.66 | 0.910845 |
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Target: 5'- gGCCGGCGUggcggcgGAGCUggagGCCcUgGCCGCu -3' miRNA: 3'- gUGGUCGCA-------CUCGA----CGGaAgUGGUGu -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 102925 | 0.66 | 0.911445 |
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Target: 5'- gCGCCccaagauCGUGGGCUGCCUgCGCaGCAu -3' miRNA: 3'- -GUGGuc-----GCACUCGACGGAaGUGgUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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