Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28831 | 3' | -56.2 | NC_006146.1 | + | 38680 | 0.69 | 0.759708 |
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Target: 5'- gGCC-GCGaGAGCUGCCUUaaagGCCugGa -3' miRNA: 3'- gUGGuCGCaCUCGACGGAAg---UGGugU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 126196 | 0.7 | 0.700576 |
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Target: 5'- gGCCAGCGgGGGCcacgGCCaccgcCGCCACAc -3' miRNA: 3'- gUGGUCGCaCUCGa---CGGaa---GUGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 137799 | 0.7 | 0.680302 |
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Target: 5'- cCGCCGGCGgccacccGGCUGCCccagaGCCGCAg -3' miRNA: 3'- -GUGGUCGCac-----UCGACGGaag--UGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 101840 | 0.71 | 0.659869 |
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Target: 5'- gGCCcgAGCGcucGGGCUGCCg-CACCACGg -3' miRNA: 3'- gUGG--UCGCa--CUCGACGGaaGUGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 115125 | 0.71 | 0.617772 |
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Target: 5'- gGCCAGCGUGGuggccucGCUGCCccuggccCGCCGCc -3' miRNA: 3'- gUGGUCGCACU-------CGACGGaa-----GUGGUGu -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 146835 | 0.72 | 0.588073 |
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Target: 5'- gCGCCAGCGUaGAGCccggGCCUggcUCgggGCCGCGu -3' miRNA: 3'- -GUGGUCGCA-CUCGa---CGGA---AG---UGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 170089 | 0.75 | 0.406482 |
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Target: 5'- gGCCGGCGgGAGggGCCggCGCCGCAg -3' miRNA: 3'- gUGGUCGCaCUCgaCGGaaGUGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 75511 | 1.08 | 0.003229 |
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Target: 5'- gCACCAGCGUGAGCUGCCUUCACCACAc -3' miRNA: 3'- -GUGGUCGCACUCGACGGAAGUGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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