Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28831 | 3' | -56.2 | NC_006146.1 | + | 137799 | 0.7 | 0.680302 |
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Target: 5'- cCGCCGGCGgccacccGGCUGCCccagaGCCGCAg -3' miRNA: 3'- -GUGGUCGCac-----UCGACGGaag--UGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 146835 | 0.72 | 0.588073 |
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Target: 5'- gCGCCAGCGUaGAGCccggGCCUggcUCgggGCCGCGu -3' miRNA: 3'- -GUGGUCGCA-CUCGa---CGGA---AG---UGGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 152541 | 0.66 | 0.878541 |
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Target: 5'- gACCAGgGUGAGCaUGCC---ACCAa- -3' miRNA: 3'- gUGGUCgCACUCG-ACGGaagUGGUgu -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 160203 | 0.66 | 0.885581 |
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Target: 5'- uCGCCAGUcUGuGCUGCCUgCAugaugcCCGCGg -3' miRNA: 3'- -GUGGUCGcACuCGACGGAaGU------GGUGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 167293 | 0.69 | 0.778607 |
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Target: 5'- gGCCGGCGgGAGggGCCggCGCCuGCAg -3' miRNA: 3'- gUGGUCGCaCUCgaCGGaaGUGG-UGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 168225 | 0.69 | 0.778607 |
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Target: 5'- gGCCGGCGgGAGggGCCggCGCCuGCAg -3' miRNA: 3'- gUGGUCGCaCUCgaCGGaaGUGG-UGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 169157 | 0.69 | 0.778607 |
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Target: 5'- gGCCGGCGgGAGggGCCggCGCCuGCAg -3' miRNA: 3'- gUGGUCGCaCUCgaCGGaaGUGG-UGU- -5' |
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| 28831 | 3' | -56.2 | NC_006146.1 | + | 170089 | 0.75 | 0.406482 |
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Target: 5'- gGCCGGCGgGAGggGCCggCGCCGCAg -3' miRNA: 3'- gUGGUCGCaCUCgaCGGaaGUGGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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