Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28839 | 3' | -57.8 | NC_006146.1 | + | 51582 | 0.69 | 0.654766 |
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Target: 5'- cGGGAGGGGCCCggg---GgaGCCa-- -3' miRNA: 3'- -UCCUCCCCGGGauaagaCgaCGGaug -5' |
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| 28839 | 3' | -57.8 | NC_006146.1 | + | 124529 | 0.7 | 0.583404 |
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Target: 5'- aGGGAcGGGGgCCUGggggagCUGCUGUCggACg -3' miRNA: 3'- -UCCU-CCCCgGGAUaa----GACGACGGa-UG- -5' |
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| 28839 | 3' | -57.8 | NC_006146.1 | + | 92084 | 0.7 | 0.583404 |
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Target: 5'- uGGGGGGcGGCCCggccugGggCUGCUGCUn-- -3' miRNA: 3'- -UCCUCC-CCGGGa-----UaaGACGACGGaug -5' |
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| 28839 | 3' | -57.8 | NC_006146.1 | + | 92234 | 0.7 | 0.571275 |
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Target: 5'- gGGGGGcGGGCCCggccuguggCUGCUGCUn-- -3' miRNA: 3'- -UCCUC-CCCGGGauaa-----GACGACGGaug -5' |
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| 28839 | 3' | -57.8 | NC_006146.1 | + | 15029 | 0.73 | 0.456807 |
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Target: 5'- aGGGAGGGGCgCCUGgccaggGCccgccggGCCUGCg -3' miRNA: 3'- -UCCUCCCCG-GGAUaaga--CGa------CGGAUG- -5' |
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| 28839 | 3' | -57.8 | NC_006146.1 | + | 102896 | 0.66 | 0.844099 |
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Target: 5'- gGGGAcgccaGGGGCCgUGUggcguggcugCgccccaagaucguggGCUGCCUGCg -3' miRNA: 3'- -UCCU-----CCCCGGgAUAa---------Ga--------------CGACGGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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