Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28846 | 3' | -54.6 | NC_006146.1 | + | 47642 | 0.68 | 0.895819 |
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Target: 5'- aGCCgUCUCuGgccgCGGCCcggccCAGCGCGGCGg -3' miRNA: 3'- -UGG-AGAG-Ca---GCUGGua---GUCGCGUUGC- -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 5737 | 0.69 | 0.882288 |
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Target: 5'- cACCUCUgGUCGcUCA--AGCGCAGCc -3' miRNA: 3'- -UGGAGAgCAGCuGGUagUCGCGUUGc -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 85709 | 0.69 | 0.860349 |
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Target: 5'- gUCUCUCGuccaUCGGCCAUgucaGGCGUGACa -3' miRNA: 3'- uGGAGAGC----AGCUGGUAg---UCGCGUUGc -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 155724 | 0.69 | 0.852618 |
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Target: 5'- gGCCgCgcaGUCGGCCuUCAGCGCuuccACGg -3' miRNA: 3'- -UGGaGag-CAGCUGGuAGUCGCGu---UGC- -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 49483 | 0.7 | 0.836567 |
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Target: 5'- gGCCgcggUGUCGGCCAggggCGGCGUGGCa -3' miRNA: 3'- -UGGaga-GCAGCUGGUa---GUCGCGUUGc -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 42911 | 0.71 | 0.746503 |
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Target: 5'- gGCCUCU-GUccCGGCUGUCAGCaGCGGCGc -3' miRNA: 3'- -UGGAGAgCA--GCUGGUAGUCG-CGUUGC- -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 44936 | 0.76 | 0.515632 |
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Target: 5'- aACCUCUCGU-GGCCAggGGCGCGGgGa -3' miRNA: 3'- -UGGAGAGCAgCUGGUagUCGCGUUgC- -5' |
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| 28846 | 3' | -54.6 | NC_006146.1 | + | 43475 | 0.66 | 0.960997 |
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Target: 5'- aGCCUcCUCGgCGGCCGUugacacCGGCuccgGCGGCGg -3' miRNA: 3'- -UGGA-GAGCaGCUGGUA------GUCG----CGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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