Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28847 | 3' | -52.5 | NC_006146.1 | + | 95551 | 0.71 | 0.871984 |
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Target: 5'- gGGCagCGUauGACAUGGACACCugGAc -3' miRNA: 3'- -UCGgaGUAggCUGUACUUGUGGugCU- -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 124272 | 0.71 | 0.871984 |
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Target: 5'- uGCCUCAUCCGgaacgGCcgGGACAUCAacaGGc -3' miRNA: 3'- uCGGAGUAGGC-----UGuaCUUGUGGUg--CU- -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 124226 | 0.71 | 0.84869 |
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Target: 5'- -uCCUgGUCCGACGUGGAgGCCAaCGc -3' miRNA: 3'- ucGGAgUAGGCUGUACUUgUGGU-GCu -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 2941 | 0.72 | 0.84051 |
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Target: 5'- gAGCUgggagUCAUCaGACAccaggcgcUGGACACCGCGAg -3' miRNA: 3'- -UCGG-----AGUAGgCUGU--------ACUUGUGGUGCU- -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 71298 | 0.72 | 0.84051 |
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Target: 5'- cGCCUCGUgCuacGCA-GAGCACCGCGGc -3' miRNA: 3'- uCGGAGUAgGc--UGUaCUUGUGGUGCU- -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 60362 | 0.73 | 0.779177 |
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Target: 5'- cAGCCUCuuugaacaggaggucUCCGAgGUGcAGCGCCugGAg -3' miRNA: 3'- -UCGGAGu--------------AGGCUgUAC-UUGUGGugCU- -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 71156 | 0.75 | 0.662088 |
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Target: 5'- uGGCCUCuUCCGAUGgguccgcguccucgGAGCACCGCGu -3' miRNA: 3'- -UCGGAGuAGGCUGUa-------------CUUGUGGUGCu -5' |
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| 28847 | 3' | -52.5 | NC_006146.1 | + | 56776 | 1.09 | 0.006776 |
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Target: 5'- gAGCCUCAUCCGACAUGAACACCACGAc -3' miRNA: 3'- -UCGGAGUAGGCUGUACUUGUGGUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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