Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2885 | 3' | -50.8 | NC_001493.1 | + | 33509 | 0.66 | 0.990651 |
Target: 5'- cACCACccacaaggcaguuGgGGUCUUgaagcagCCGUcGUGCACc -3' miRNA: 3'- -UGGUG-------------UgCCAGAAaaa----GGCA-CACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 66980 | 0.66 | 0.986335 |
Target: 5'- aGCCgACGCGGUgaUUUUCgaucauaugcuCGUGaUGCACu -3' miRNA: 3'- -UGG-UGUGCCAgaAAAAG-----------GCAC-ACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 35639 | 0.66 | 0.984537 |
Target: 5'- aACUACucuACGGUCUcg--CCGggGUGUACg -3' miRNA: 3'- -UGGUG---UGCCAGAaaaaGGCa-CACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 47368 | 0.67 | 0.980408 |
Target: 5'- uCCACGCGGUUgcccacaUCCGUGaucGCAg -3' miRNA: 3'- uGGUGUGCCAGaaaa---AGGCACa--CGUg -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 73622 | 0.67 | 0.979492 |
Target: 5'- gACCAcCGCGGUCcccccugcgCUGcgGUGCACa -3' miRNA: 3'- -UGGU-GUGCCAGaaaaa----GGCa-CACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 85649 | 0.67 | 0.978059 |
Target: 5'- uGCCGCgGCGGUCcca--CCG-GUGUACa -3' miRNA: 3'- -UGGUG-UGCCAGaaaaaGGCaCACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 110463 | 0.68 | 0.966563 |
Target: 5'- gACCcguCACGGUaaaggagagUUUUUUCCGcgGUGCAUu -3' miRNA: 3'- -UGGu--GUGCCA---------GAAAAAGGCa-CACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 73231 | 0.68 | 0.963126 |
Target: 5'- uCUugACGGUCUUUUcggCCGUG-GC-Ca -3' miRNA: 3'- uGGugUGCCAGAAAAa--GGCACaCGuG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 38143 | 0.68 | 0.955529 |
Target: 5'- gACCGCGauCGGgCUUcgUCCGgGUGCAUa -3' miRNA: 3'- -UGGUGU--GCCaGAAaaAGGCaCACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 82064 | 0.7 | 0.914912 |
Target: 5'- gGCCACGCGGUCUUUgaucgCC-UGgcugGcCACg -3' miRNA: 3'- -UGGUGUGCCAGAAAaa---GGcACa---C-GUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 60075 | 0.7 | 0.914298 |
Target: 5'- uACCACACGGUCUcg-UUCGgagGUgggacggugacgaGCACg -3' miRNA: 3'- -UGGUGUGCCAGAaaaAGGCa--CA-------------CGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 57553 | 0.7 | 0.908655 |
Target: 5'- uCCGCACGGUCacgg-CCucGUGCACg -3' miRNA: 3'- uGGUGUGCCAGaaaaaGGcaCACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 43884 | 0.74 | 0.756912 |
Target: 5'- gGCCGCgACGGUCUgaggggCCGUcaccagGUGCGCu -3' miRNA: 3'- -UGGUG-UGCCAGAaaaa--GGCA------CACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 11490 | 0.97 | 0.045275 |
Target: 5'- cACCACACGGUCUUUUU-CGUGUGCACg -3' miRNA: 3'- -UGGUGUGCCAGAAAAAgGCACACGUG- -5' |
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2885 | 3' | -50.8 | NC_001493.1 | + | 127045 | 1.11 | 0.005803 |
Target: 5'- cACCACACGGUCUUUUUCCGUGUGCACg -3' miRNA: 3'- -UGGUGUGCCAGAAAAAGGCACACGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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