miRNA: 3'- -UGCCC--------UCGACuCCG-ACU-------UGCACUGGu -5'
miRNA: 3'- uGCCCUCGA---------CuCCGACUugCaCUGgU- -5'
miRNA: 3'- -UGCCCUCGaCUCCGacuugcaCUGGU- -5'
Results 81 - 83 of 83 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28857 | 3' | -57.1 | NC_006146.1 | + | 169649 | 0.66 | 0.893098 |
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Target: 5'- gGCGGGgagaauacAGCUG-GGCgUGGcgggcgcGCGUGGCCc -3' miRNA: 3'- -UGCCC--------UCGACuCCG-ACU-------UGCACUGGu -5' |
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| 28857 | 3' | -57.1 | NC_006146.1 | + | 44336 | 0.66 | 0.893755 |
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Target: 5'- cCGGGGGCUucugcacagGuGGCUGAugGgGACgAg -3' miRNA: 3'- uGCCCUCGA---------CuCCGACUugCaCUGgU- -5' |
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| 28857 | 3' | -57.1 | NC_006146.1 | + | 56245 | 0.66 | 0.893755 |
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Target: 5'- cGCGGGGGCgGAGGCgg-----GGCCGu -3' miRNA: 3'- -UGCCCUCGaCUCCGacuugcaCUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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