Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28859 | 3' | -50.2 | NC_006146.1 | + | 141298 | 0.66 | 0.994843 |
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Target: 5'- gGGCGGCUgggggUCGG--GCUGGCCu- -3' miRNA: 3'- -UCGCUGAagua-AGUCuaUGACCGGug -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 147454 | 0.66 | 0.994843 |
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Target: 5'- gGGCGGCUgggggUCGG--GCUGGCCu- -3' miRNA: 3'- -UCGCUGAagua-AGUCuaUGACCGGug -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 12031 | 0.66 | 0.995586 |
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Target: 5'- cGGCaGCUaCGUggCGGGUGC-GGCCACc -3' miRNA: 3'- -UCGcUGAaGUAa-GUCUAUGaCCGGUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 10392 | 0.66 | 0.995586 |
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Target: 5'- cGCGGC-UCAggguGGUGCUGuGCUACa -3' miRNA: 3'- uCGCUGaAGUaaguCUAUGAC-CGGUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 76711 | 0.66 | 0.995586 |
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Target: 5'- cGGCGACUUCAU--GGAcACgcucgaGGCCGa -3' miRNA: 3'- -UCGCUGAAGUAagUCUaUGa-----CCGGUg -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 160318 | 0.66 | 0.996239 |
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Target: 5'- --aGAUUUCAggaAGAcgcucagGCUGGCCACg -3' miRNA: 3'- ucgCUGAAGUaagUCUa------UGACCGGUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 130299 | 0.66 | 0.996239 |
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Target: 5'- cAGCGGCggCAUgCGGAgguucGGCCGCc -3' miRNA: 3'- -UCGCUGaaGUAaGUCUauga-CCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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