Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28859 | 3' | -50.2 | NC_006146.1 | + | 42113 | 0.69 | 0.969951 |
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Target: 5'- uGUGGCcgaUCAcgcUUCGGcccUGCUGGCCACa -3' miRNA: 3'- uCGCUGa--AGU---AAGUCu--AUGACCGGUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 149594 | 0.69 | 0.969333 |
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Target: 5'- gGGCGGCgggaga-GGAgGCUGGCCGCc -3' miRNA: 3'- -UCGCUGaaguaagUCUaUGACCGGUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 115503 | 0.7 | 0.947315 |
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Target: 5'- gGGCGACUaCGccguGAUGCUGGCCuCg -3' miRNA: 3'- -UCGCUGAaGUaaguCUAUGACCGGuG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 74487 | 0.71 | 0.915573 |
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Target: 5'- gAGCGGCgcugUGUUCAGGaGCUGGCaCAUc -3' miRNA: 3'- -UCGCUGaa--GUAAGUCUaUGACCG-GUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 111905 | 0.72 | 0.889226 |
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Target: 5'- gAGCagGGCUUCGUUCAGGgacgcguucuUGgUGGCCAUc -3' miRNA: 3'- -UCG--CUGAAGUAAGUCU----------AUgACCGGUG- -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 71582 | 0.72 | 0.882011 |
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Target: 5'- gAGCGGCUUCcgcagCAGGU-CUGGCCc- -3' miRNA: 3'- -UCGCUGAAGuaa--GUCUAuGACCGGug -5' |
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| 28859 | 3' | -50.2 | NC_006146.1 | + | 45776 | 1.11 | 0.00731 |
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Target: 5'- cAGCGACUUCAUUCAGAUACUGGCCACg -3' miRNA: 3'- -UCGCUGAAGUAAGUCUAUGACCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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