Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2886 | 5' | -56 | NC_001493.1 | + | 81453 | 0.67 | 0.828426 |
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Target: 5'- cCACG-GUcgG-GGCgUCAGUGUCGCCc -3' miRNA: 3'- -GUGCuCGuaCgUCG-AGUCACAGCGGa -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 63863 | 0.69 | 0.683652 |
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Target: 5'- cCGCGGGCGUGCcguGGUgauauucgCGGUGUCGUUg -3' miRNA: 3'- -GUGCUCGUACG---UCGa-------GUCACAGCGGa -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 125211 | 0.7 | 0.642005 |
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Target: 5'- uCGCGuGCG-GCAGUUgAGUgGUCGCCg -3' miRNA: 3'- -GUGCuCGUaCGUCGAgUCA-CAGCGGa -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 9656 | 0.7 | 0.642005 |
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Target: 5'- uCGCGuGCG-GCAGUUgAGUgGUCGCCg -3' miRNA: 3'- -GUGCuCGUaCGUCGAgUCA-CAGCGGa -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 120722 | 0.72 | 0.519278 |
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Target: 5'- -cCGAGCGUGCAGC-CGGUGgacuuccucgucaggCGCCc -3' miRNA: 3'- guGCUCGUACGUCGaGUCACa--------------GCGGa -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 47738 | 0.74 | 0.413917 |
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Target: 5'- gCACGAGCA-GCGGCUUuGUGaCGCCc -3' miRNA: 3'- -GUGCUCGUaCGUCGAGuCACaGCGGa -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 5167 | 0.74 | 0.405955 |
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Target: 5'- -cCGAGCGUGCAGC-CGGUGgacuuccucgucaggCGCCUc -3' miRNA: 3'- guGCUCGUACGUCGaGUCACa--------------GCGGA- -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 128725 | 1.07 | 0.00294 |
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Target: 5'- cCACGAGCAUGCAGCUCAGUGUCGCCUu -3' miRNA: 3'- -GUGCUCGUACGUCGAGUCACAGCGGA- -5' |
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| 2886 | 5' | -56 | NC_001493.1 | + | 13171 | 1.07 | 0.00294 |
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Target: 5'- cCACGAGCAUGCAGCUCAGUGUCGCCUu -3' miRNA: 3'- -GUGCUCGUACGUCGAGUCACAGCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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