miRNA: 3'- -GUCA----UUCGGCUACaaa----UGGAGC- -5'
miRNA: 3'- gUCAUUCGGCUacaaAUGGAGC- -5'
miRNA: 3'- -GUCAU-----UCGGCUACAAaUGGaGC- -5'
miRNA: 3'- -GUCA----UUCGGCUACaaa-UGGAgC- -5'
miRNA: 3'- gUCAU--UCGGCuacaa-AUGGAGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28867 | 5' | -41 | NC_006146.1 | + | 4421 | 0.66 | 1 |
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Target: 5'- aCGGUccagGGGCUGGUGaaaagcgGCCUCGu -3' miRNA: 3'- -GUCA----UUCGGCUACaaa----UGGAGC- -5' |
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| 28867 | 5' | -41 | NC_006146.1 | + | 39651 | 0.69 | 1 |
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Target: 5'- aGGUGGGCUGAgac-UGCCUUGg -3' miRNA: 3'- gUCAUUCGGCUacaaAUGGAGC- -5' |
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| 28867 | 5' | -41 | NC_006146.1 | + | 95739 | 0.68 | 1 |
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Target: 5'- cCAGUGgggggGGCUGGUGUUgGCCcCGg -3' miRNA: 3'- -GUCAU-----UCGGCUACAAaUGGaGC- -5' |
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| 28867 | 5' | -41 | NC_006146.1 | + | 112510 | 0.68 | 1 |
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Target: 5'- gCGGUccagGGGCCGGUGgaggGCCUgGg -3' miRNA: 3'- -GUCA----UUCGGCUACaaa-UGGAgC- -5' |
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| 28867 | 5' | -41 | NC_006146.1 | + | 167925 | 0.66 | 1 |
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Target: 5'- aGGUAcuGGCCGuacucgUACCUCGc -3' miRNA: 3'- gUCAU--UCGGCuacaa-AUGGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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