Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2887 | 5' | -52 | NC_001493.1 | + | 1003 | 0.66 | 0.981983 |
Target: 5'- gGCGAUgAUGAuggcuUCAugACGUG-CGCCGGu -3' miRNA: 3'- -UGCUAgUGCU-----AGU--UGCACuGCGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 116557 | 0.66 | 0.981983 |
Target: 5'- gGCGAUgAUGAuggcuUCAugACGUG-CGCCGGu -3' miRNA: 3'- -UGCUAgUGCU-----AGU--UGCACuGCGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 32421 | 0.66 | 0.979804 |
Target: 5'- cACGAUCGCGggUAugGagGACGUCa-- -3' miRNA: 3'- -UGCUAGUGCuaGUugCa-CUGCGGcuu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 31117 | 0.66 | 0.979804 |
Target: 5'- -gGAUCGCGG---ACGUGAaggcCGCCGAc -3' miRNA: 3'- ugCUAGUGCUaguUGCACU----GCGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 55174 | 0.66 | 0.977435 |
Target: 5'- -gGGUCaACGAUCcGCGgugaGACGCCGc- -3' miRNA: 3'- ugCUAG-UGCUAGuUGCa---CUGCGGCuu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 1953 | 0.66 | 0.977435 |
Target: 5'- gGCGGUgAcccaagguCGAUCGACGUGGCaaaGCUGAc -3' miRNA: 3'- -UGCUAgU--------GCUAGUUGCACUG---CGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 117507 | 0.66 | 0.977435 |
Target: 5'- gGCGGUgAcccaagguCGAUCGACGUGGCaaaGCUGAc -3' miRNA: 3'- -UGCUAgU--------GCUAGUUGCACUG---CGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 39479 | 0.67 | 0.970327 |
Target: 5'- cUGAUCGgGAUCGgcgucagcgucacccGCGUcACGCCGGAu -3' miRNA: 3'- uGCUAGUgCUAGU---------------UGCAcUGCGGCUU- -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 47743 | 0.67 | 0.965896 |
Target: 5'- cGCGcgCACGAgcagCGGCuuuGUGACGCCc-- -3' miRNA: 3'- -UGCuaGUGCUa---GUUG---CACUGCGGcuu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 89572 | 0.68 | 0.931599 |
Target: 5'- cACGGUCACGAgaccgaacgUCGugACG-GGCGCCGu- -3' miRNA: 3'- -UGCUAGUGCU---------AGU--UGCaCUGCGGCuu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 65541 | 0.68 | 0.931599 |
Target: 5'- cUGAUUgAUGAUCAACaUGACGCCGu- -3' miRNA: 3'- uGCUAG-UGCUAGUUGcACUGCGGCuu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 48345 | 0.69 | 0.920504 |
Target: 5'- cUGAaacgCACGGUgGACGcGACGCCGGc -3' miRNA: 3'- uGCUa---GUGCUAgUUGCaCUGCGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 118866 | 0.69 | 0.920504 |
Target: 5'- aGCGAgugucgacUCuGCGAUgGAUGUgGACGCCGAGu -3' miRNA: 3'- -UGCU--------AG-UGCUAgUUGCA-CUGCGGCUU- -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 3312 | 0.69 | 0.920504 |
Target: 5'- aGCGAgugucgacUCuGCGAUgGAUGUgGACGCCGAGu -3' miRNA: 3'- -UGCU--------AG-UGCUAgUUGCA-CUGCGGCUU- -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 39649 | 0.7 | 0.888316 |
Target: 5'- gGCGugaCGCGggUGACGcUGACGCCGAu -3' miRNA: 3'- -UGCua-GUGCuaGUUGC-ACUGCGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 64882 | 0.7 | 0.873719 |
Target: 5'- cCGGUCGCGAUgAACG-GAC-CCGAc -3' miRNA: 3'- uGCUAGUGCUAgUUGCaCUGcGGCUu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 24689 | 0.72 | 0.797348 |
Target: 5'- uACGGUgccgugacaCACGAUCAACGUGAauCGCCu-- -3' miRNA: 3'- -UGCUA---------GUGCUAGUUGCACU--GCGGcuu -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 12252 | 0.74 | 0.666213 |
Target: 5'- aGCGAUCGCGGgcgcgguccauaUCAAUGUGAgGUCGGGg -3' miRNA: 3'- -UGCUAGUGCU------------AGUUGCACUgCGGCUU- -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 127806 | 0.74 | 0.666213 |
Target: 5'- aGCGAUCGCGGgcgcgguccauaUCAAUGUGAgGUCGGGg -3' miRNA: 3'- -UGCUAGUGCU------------AGUUGCACUgCGGCUU- -5' |
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2887 | 5' | -52 | NC_001493.1 | + | 14252 | 1.06 | 0.009929 |
Target: 5'- cACGAUCACGAUCAACGUGACGCCGAAc -3' miRNA: 3'- -UGCUAGUGCUAGUUGCACUGCGGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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