Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28879 | 5' | -49.7 | NC_006146.1 | + | 43067 | 0.66 | 0.994512 |
Target: 5'- gGUUgagGGGUGCCAGCgACUGGc---- -3' miRNA: 3'- aUAGaa-UCCACGGUCG-UGAUCacuua -5' |
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28879 | 5' | -49.7 | NC_006146.1 | + | 95071 | 0.67 | 0.987142 |
Target: 5'- -cUCgu-GGUGgCAGCugUGGUGGGg -3' miRNA: 3'- auAGaauCCACgGUCGugAUCACUUa -5' |
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28879 | 5' | -49.7 | NC_006146.1 | + | 139464 | 0.69 | 0.973792 |
Target: 5'- -----gGGGUGCCuGCACUGGaGAAg -3' miRNA: 3'- auagaaUCCACGGuCGUGAUCaCUUa -5' |
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28879 | 5' | -49.7 | NC_006146.1 | + | 48319 | 0.75 | 0.764788 |
Target: 5'- cGUCggAGGUGCUGGCGgUGGUGGGc -3' miRNA: 3'- aUAGaaUCCACGGUCGUgAUCACUUa -5' |
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28879 | 5' | -49.7 | NC_006146.1 | + | 30418 | 1.05 | 0.016032 |
Target: 5'- uUAUCUUAGGUGCCAGCACUAGUGAAUa -3' miRNA: 3'- -AUAGAAUCCACGGUCGUGAUCACUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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