Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28882 | 5' | -42.6 | NC_006146.1 | + | 87952 | 0.66 | 1 |
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Target: 5'- -uUUGUG-UGGAAAUGAGAGCcuuccaaacGAGg -3' miRNA: 3'- uuGACAUaACCUUUACUCUUGaa-------CUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 51203 | 0.66 | 1 |
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Target: 5'- aAGCUGU---GGAGcAUGGGAgguuGCUUGGGg -3' miRNA: 3'- -UUGACAuaaCCUU-UACUCU----UGAACUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 161698 | 0.67 | 1 |
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Target: 5'- cGugUGUGaUGGGGAgcuccuggGAGAagGCUUGGGg -3' miRNA: 3'- -UugACAUaACCUUUa-------CUCU--UGAACUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 103342 | 0.67 | 1 |
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Target: 5'- uGAUUGg--UGGAGAUGGGAGCg---- -3' miRNA: 3'- -UUGACauaACCUUUACUCUUGaacuc -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 141113 | 0.66 | 1 |
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Target: 5'- -cUUGUAUcGGAGAU-AGGACUUGAu -3' miRNA: 3'- uuGACAUAaCCUUUAcUCUUGAACUc -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 125994 | 0.69 | 0.999997 |
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Target: 5'- cGGCUGcgaggcUUGGGAGUGGGGGCggUGGGc -3' miRNA: 3'- -UUGACau----AACCUUUACUCUUGa-ACUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 89573 | 0.69 | 0.999995 |
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Target: 5'- -uUUGgagggUGGGGcgGAGGACUUGGGu -3' miRNA: 3'- uuGACaua--ACCUUuaCUCUUGAACUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 153520 | 0.69 | 0.999993 |
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Target: 5'- aGGCUGUGgauggagUGGGGggcGUGGGGcuGCUUGGGa -3' miRNA: 3'- -UUGACAUa------ACCUU---UACUCU--UGAACUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 115015 | 0.7 | 0.999963 |
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Target: 5'- gAGCUGg--UGGAGcgGGGAGCagGGGg -3' miRNA: 3'- -UUGACauaACCUUuaCUCUUGaaCUC- -5' |
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| 28882 | 5' | -42.6 | NC_006146.1 | + | 29821 | 1.07 | 0.076458 |
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Target: 5'- aAACUGUAUUGGAAAUGAGAACUUGAGc -3' miRNA: 3'- -UUGACAUAACCUUUACUCUUGAACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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