Results 1 - 20 of 160 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28883 | 5' | -63.5 | NC_006146.1 | + | 287 | 0.71 | 0.340049 |
Target: 5'- -cCGGGAagaCCCGGGGGCg-GGCCcGGc -3' miRNA: 3'- cuGUCCUg--GGGCCCCCGagUCGGuCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 580 | 0.66 | 0.608524 |
Target: 5'- gGAgAGGGgCC-GGGGGCgCGGCCcGGc -3' miRNA: 3'- -CUgUCCUgGGgCCCCCGaGUCGGuCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 921 | 0.69 | 0.434187 |
Target: 5'- -cCGGGACCCCGGGcGCgc-GCCGGc -3' miRNA: 3'- cuGUCCUGGGGCCCcCGaguCGGUCc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 1218 | 0.7 | 0.377422 |
Target: 5'- -cCGGGAagaCCCGGGGGCg-GGCCAc- -3' miRNA: 3'- cuGUCCUg--GGGCCCCCGagUCGGUcc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 1332 | 0.66 | 0.626859 |
Target: 5'- cGACGcgcgcucGGGCCCCGGGGaagccccccGCUC-GCCccucGGGu -3' miRNA: 3'- -CUGU-------CCUGGGGCCCC---------CGAGuCGG----UCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 1511 | 0.66 | 0.608524 |
Target: 5'- gGAgAGGGgCC-GGGGGCgCGGCCcGGc -3' miRNA: 3'- -CUgUCCUgGGgCCCCCGaGUCGGuCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 1853 | 0.69 | 0.434187 |
Target: 5'- -cCGGGACCCCGGGcGCgc-GCCGGc -3' miRNA: 3'- cuGUCCUGGGGCCCcCGaguCGGUCc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 2150 | 0.7 | 0.377422 |
Target: 5'- -cCGGGAagaCCCGGGGGCg-GGCCAc- -3' miRNA: 3'- cuGUCCUg--GGGCCCCCGagUCGGUcc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 2264 | 0.66 | 0.626859 |
Target: 5'- cGACGcgcgcucGGGCCCCGGGGaagccccccGCUC-GCCccucGGGu -3' miRNA: 3'- -CUGU-------CCUGGGGCCCC---------CGAGuCGG----UCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 2443 | 0.66 | 0.608524 |
Target: 5'- gGAgAGGGgCC-GGGGGCgCGGCCcGGc -3' miRNA: 3'- -CUgUCCUgGGgCCCCCGaGUCGGuCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 2785 | 0.69 | 0.434187 |
Target: 5'- -cCGGGACCCCGGGcGCgc-GCCGGc -3' miRNA: 3'- cuGUCCUGGGGCCCcCGaguCGGUCc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 3082 | 0.7 | 0.377422 |
Target: 5'- -cCGGGAagaCCCGGGGGCg-GGCCAc- -3' miRNA: 3'- cuGUCCUg--GGGCCCCCGagUCGGUcc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 3196 | 0.66 | 0.626859 |
Target: 5'- cGACGcgcgcucGGGCCCCGGGGaagccccccGCUC-GCCccucGGGu -3' miRNA: 3'- -CUGU-------CCUGGGGCCCC---------CGAGuCGG----UCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 3375 | 0.66 | 0.608524 |
Target: 5'- gGAgAGGGgCC-GGGGGCgCGGCCcGGc -3' miRNA: 3'- -CUgUCCUgGGgCCCCCGaGUCGGuCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 3717 | 0.69 | 0.434187 |
Target: 5'- -cCGGGACCCCGGGcGCgc-GCCGGc -3' miRNA: 3'- cuGUCCUGGGGCCCcCGaguCGGUCc -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 3722 | 0.76 | 0.152788 |
Target: 5'- aGACAGGGuCUCUGGGGGCcgcguggcccuUCAGcCCGGGg -3' miRNA: 3'- -CUGUCCU-GGGGCCCCCG-----------AGUC-GGUCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 4363 | 0.66 | 0.618169 |
Target: 5'- cGACGuGGccacgGCCCCGcGGGCUCc-CCAGGc -3' miRNA: 3'- -CUGU-CC-----UGGGGCcCCCGAGucGGUCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 5064 | 0.66 | 0.589286 |
Target: 5'- uGCGGGGCUggCCGuuGGaGGCguccacgCGGCCAGGg -3' miRNA: 3'- cUGUCCUGG--GGC--CC-CCGa------GUCGGUCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 13107 | 0.66 | 0.589286 |
Target: 5'- gGACcuGGCCCuguuuguggaCGGGGGCUCccgGGCCcacgAGGa -3' miRNA: 3'- -CUGucCUGGG----------GCCCCCGAG---UCGG----UCC- -5' |
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28883 | 5' | -63.5 | NC_006146.1 | + | 13419 | 0.67 | 0.570158 |
Target: 5'- -uCAGGGugccuCCCCGGGucccaGGC-CAGCCGGa -3' miRNA: 3'- cuGUCCU-----GGGGCCC-----CCGaGUCGGUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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