Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28884 | 5' | -48.3 | NC_006146.1 | + | 55583 | 0.66 | 0.99828 |
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Target: 5'- gUCCGggGUGCcguaGGUCUGGUCc -3' miRNA: 3'- -GGGUgaCACGua--CCAGACCAGa -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 94983 | 0.66 | 0.997921 |
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Target: 5'- aCCCACUGgccagGCAUGGg--GGgcagCUg -3' miRNA: 3'- -GGGUGACa----CGUACCagaCCa---GA- -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 100828 | 0.68 | 0.992171 |
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Target: 5'- cCCCAUUucucaGUGCGgacGGUCgGGUCUg -3' miRNA: 3'- -GGGUGA-----CACGUa--CCAGaCCAGA- -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 33769 | 0.68 | 0.990956 |
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Target: 5'- gUCCGCUGguccggguggGCGUGGUCcgaugGGUCc -3' miRNA: 3'- -GGGUGACa---------CGUACCAGa----CCAGa -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 33955 | 0.68 | 0.990956 |
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Target: 5'- gUCCGCUGguccggguggGCGUGGUCcgcugGGUCc -3' miRNA: 3'- -GGGUGACa---------CGUACCAGa----CCAGa -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 64850 | 0.7 | 0.972274 |
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Target: 5'- gCCGCcucagGUGCAgaagGGUCgGGUCUg -3' miRNA: 3'- gGGUGa----CACGUa---CCAGaCCAGA- -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 73080 | 0.73 | 0.90452 |
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Target: 5'- aCCACUG-GCAUGGagUGGUUg -3' miRNA: 3'- gGGUGACaCGUACCagACCAGa -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 63641 | 0.81 | 0.504169 |
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Target: 5'- uCCCGCUGUGCcugaaggugAUGGUgCUGGUCUc -3' miRNA: 3'- -GGGUGACACG---------UACCA-GACCAGA- -5' |
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| 28884 | 5' | -48.3 | NC_006146.1 | + | 29489 | 1.05 | 0.019658 |
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Target: 5'- gCCCACUGUGCAUGGUCUGGUCUg -3' miRNA: 3'- -GGGUGACACGUACCAGACCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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