Results 1 - 20 of 42 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28887 | 3' | -50.2 | NC_006146.1 | + | 156516 | 0.66 | 0.997625 |
Target: 5'- -cGCg--GCCCGGAGGGg---AGCGGg -3' miRNA: 3'- gaCGauuUGGGCCUCUUagcaUCGUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 107366 | 0.66 | 0.997625 |
Target: 5'- -aGCauuACCCGGcuguaaaagucAGAAaagCGUAGCAGg -3' miRNA: 3'- gaCGauuUGGGCC-----------UCUUa--GCAUCGUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 39990 | 0.66 | 0.997227 |
Target: 5'- gCUGCUGcGGCCCGuAGuggccggugugggCGUAGCAGg -3' miRNA: 3'- -GACGAU-UUGGGCcUCuua----------GCAUCGUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 147660 | 0.66 | 0.996667 |
Target: 5'- -gGCUGGACUCGGGGcga-GUGGaCGGg -3' miRNA: 3'- gaCGAUUUGGGCCUCuuagCAUC-GUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 156893 | 0.66 | 0.996667 |
Target: 5'- -gGCUGGACUCGGGGcga-GUGGaCGGg -3' miRNA: 3'- gaCGAUUUGGGCCUCuuagCAUC-GUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 150737 | 0.66 | 0.996667 |
Target: 5'- -gGCUGGACUCGGGGcga-GUGGaCGGg -3' miRNA: 3'- gaCGAUUUGGGCCUCuuagCAUC-GUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 153815 | 0.66 | 0.996667 |
Target: 5'- -gGCUGGACUCGGGGcga-GUGGaCGGg -3' miRNA: 3'- gaCGAUUUGGGCCUCuuagCAUC-GUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 144582 | 0.66 | 0.996667 |
Target: 5'- -gGCUGGACUCGGGGcga-GUGGaCGGg -3' miRNA: 3'- gaCGAUUUGGGCCUCuuagCAUC-GUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 141504 | 0.66 | 0.996667 |
Target: 5'- -gGCUGGACUCGGGGcga-GUGGaCGGg -3' miRNA: 3'- gaCGAUUUGGGCCUCuuagCAUC-GUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 157833 | 0.66 | 0.996667 |
Target: 5'- -cGCUGGcCCCGGAGGGgacgGGCAc -3' miRNA: 3'- gaCGAUUuGGGCCUCUUagcaUCGUc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 143302 | 0.66 | 0.996612 |
Target: 5'- ---gUGGACCCGGAGAGUCuGUcuuggaaGGCGu -3' miRNA: 3'- gacgAUUUGGGCCUCUUAG-CA-------UCGUc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 52350 | 0.66 | 0.99608 |
Target: 5'- aUGCUGGAUCUGGAGAuUCu--GCAc -3' miRNA: 3'- gACGAUUUGGGCCUCUuAGcauCGUc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 46077 | 0.66 | 0.99608 |
Target: 5'- gCUGCUGcagGGCCuCGGGGGcgaagcaccGUCGcgcgAGCAGc -3' miRNA: 3'- -GACGAU---UUGG-GCCUCU---------UAGCa---UCGUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 50205 | 0.66 | 0.99608 |
Target: 5'- -gGgUAGGCCCGGGGggUgaGUGGUc- -3' miRNA: 3'- gaCgAUUUGGGCCUCuuAg-CAUCGuc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 92482 | 0.66 | 0.99608 |
Target: 5'- aUGU---GCCCGGGGggUUGaAGguGg -3' miRNA: 3'- gACGauuUGGGCCUCuuAGCaUCguC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 8050 | 0.66 | 0.995412 |
Target: 5'- -cGCUGGACCCGGcccuGGGggUGUcuGGCAa -3' miRNA: 3'- gaCGAUUUGGGCC----UCUuaGCA--UCGUc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 126031 | 0.66 | 0.995412 |
Target: 5'- gCUGCUGGGCCgGGAGGGacugggcucccUUG-GGUAGg -3' miRNA: 3'- -GACGAUUUGGgCCUCUU-----------AGCaUCGUC- -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 121349 | 0.66 | 0.995412 |
Target: 5'- aUGCUGGGCCUGGccgg-CGUGGCc- -3' miRNA: 3'- gACGAUUUGGGCCucuuaGCAUCGuc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 77998 | 0.67 | 0.993795 |
Target: 5'- uCUGuCUGGACagcaCGGGGggUgGUGGCc- -3' miRNA: 3'- -GAC-GAUUUGg---GCCUCuuAgCAUCGuc -5' |
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28887 | 3' | -50.2 | NC_006146.1 | + | 43581 | 0.67 | 0.993795 |
Target: 5'- -aGCguu-CCCGGGGAG-CGgGGCGGg -3' miRNA: 3'- gaCGauuuGGGCCUCUUaGCaUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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