Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28887 | 5' | -54 | NC_006146.1 | + | 64534 | 0.66 | 0.970907 |
Target: 5'- aGCUGUUUCacaaaguuguAGUCUCGGGUGUugGGCa -3' miRNA: 3'- cUGACGAAGc---------UUAGGGCCCACA--UCGc -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 142377 | 0.66 | 0.957684 |
Target: 5'- -uCUGCUaCGAuUCuCCGGGUuUAGCa -3' miRNA: 3'- cuGACGAaGCUuAG-GGCCCAcAUCGc -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 55942 | 0.67 | 0.951397 |
Target: 5'- gGGCUGCUgcgugccccccgccaUCGccgCCCaGGG-GUAGCGg -3' miRNA: 3'- -CUGACGA---------------AGCuuaGGG-CCCaCAUCGC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 155792 | 0.68 | 0.920189 |
Target: 5'- uGGCUGUguguagUGugugCCUGGGUGUGGUGg -3' miRNA: 3'- -CUGACGaa----GCuua-GGGCCCACAUCGC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 152714 | 0.68 | 0.920189 |
Target: 5'- uGGCUGUguguagUGugugCCUGGGUGUGGUGg -3' miRNA: 3'- -CUGACGaa----GCuua-GGGCCCACAUCGC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 149637 | 0.68 | 0.920189 |
Target: 5'- uGGCUGUguguagUGugugCCUGGGUGUGGUGg -3' miRNA: 3'- -CUGACGaa----GCuua-GGGCCCACAUCGC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 146559 | 0.68 | 0.920189 |
Target: 5'- uGGCUGUguguagUGugugCCUGGGUGUGGUGg -3' miRNA: 3'- -CUGACGaa----GCuua-GGGCCCACAUCGC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 143481 | 0.68 | 0.920189 |
Target: 5'- uGGCUGUguguagUGugugCCUGGGUGUGGUGg -3' miRNA: 3'- -CUGACGaa----GCuua-GGGCCCACAUCGC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 138795 | 0.68 | 0.902165 |
Target: 5'- gGAgaGCUUCGGAUgggCCCGGGguugGUgAGCu -3' miRNA: 3'- -CUgaCGAAGCUUA---GGGCCCa---CA-UCGc -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 37181 | 0.71 | 0.810111 |
Target: 5'- -cUUGCUUUGAAUguggCCCGGGUG-GGCa -3' miRNA: 3'- cuGACGAAGCUUA----GGGCCCACaUCGc -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 60945 | 0.74 | 0.654093 |
Target: 5'- aGACUGCUuucaUUGAggCCCGGGgGUGGgGg -3' miRNA: 3'- -CUGACGA----AGCUuaGGGCCCaCAUCgC- -5' |
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28887 | 5' | -54 | NC_006146.1 | + | 28558 | 1.08 | 0.005967 |
Target: 5'- uGACUGCUUCGAAUCCCGGGUGUAGCGc -3' miRNA: 3'- -CUGACGAAGCUUAGGGCCCACAUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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