Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28890 | 3' | -43.4 | NC_006146.1 | + | 53484 | 0.66 | 0.999999 |
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Target: 5'- cGUCGgUCagGGCCgGggGUGCGGu -3' miRNA: 3'- -CAGCaAG--UCGGaCuaCACGCU- -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 93572 | 0.66 | 0.999998 |
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Target: 5'- cUCGUccuucaccaUCAGCCUGGgcaUGcGCGAc -3' miRNA: 3'- cAGCA---------AGUCGGACU---ACaCGCU- -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 87431 | 0.67 | 0.999993 |
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Target: 5'- uGUgGUUCuGCCUGAga-GCGAc -3' miRNA: 3'- -CAgCAAGuCGGACUacaCGCU- -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 95412 | 0.68 | 0.999959 |
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Target: 5'- gGUUGUaCAGCCaccgGAUGUGUGu -3' miRNA: 3'- -CAGCAaGUCGGa---CUACACGCu -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 67605 | 0.7 | 0.999768 |
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Target: 5'- -gCGUUUGGCCUccgGUGCGAg -3' miRNA: 3'- caGCAAGUCGGAcuaCACGCU- -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 37525 | 0.7 | 0.999615 |
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Target: 5'- uGUC-UUUGGUCUGGUGUGUGGg -3' miRNA: 3'- -CAGcAAGUCGGACUACACGCU- -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 74623 | 0.72 | 0.997332 |
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Target: 5'- -aCGUgCAGCCUGAUGcgGCGc -3' miRNA: 3'- caGCAaGUCGGACUACa-CGCu -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 22700 | 0.75 | 0.984947 |
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Target: 5'- -aUGUUCcugGGCCUGAUGUGCu- -3' miRNA: 3'- caGCAAG---UCGGACUACACGcu -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 28170 | 1.07 | 0.052061 |
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Target: 5'- aGUCGUUCAGCCUGAUGUGCGAu -3' miRNA: 3'- -CAGCAAGUCGGACUACACGCU- -5' |
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| 28890 | 3' | -43.4 | NC_006146.1 | + | 27978 | 1.07 | 0.052061 |
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Target: 5'- aGUCGUUCAGCCUGAUGUGCGAu -3' miRNA: 3'- -CAGCAAGUCGGACUACACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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