Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28892 | 3' | -48 | NC_006146.1 | + | 49960 | 0.66 | 0.999599 |
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Target: 5'- gGUUCuUGGAguGGGCGAUGg--UCCUCUu -3' miRNA: 3'- -UAAG-AUCU--UCCGCUAUgaaAGGAGGu -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 130970 | 0.66 | 0.99922 |
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Target: 5'- -gUC-AGGAGGCgcccaGAUACUUcccccacccUCCUCCGu -3' miRNA: 3'- uaAGaUCUUCCG-----CUAUGAA---------AGGAGGU- -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 46030 | 0.67 | 0.998822 |
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Target: 5'- -gUCuUGGAAGGCGAcggggcCCUCCAg -3' miRNA: 3'- uaAG-AUCUUCCGCUaugaaaGGAGGU- -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 71261 | 0.68 | 0.997017 |
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Target: 5'- ----cGGggGGCGGccucUACgccUUCCUCCGg -3' miRNA: 3'- uaagaUCuuCCGCU----AUGa--AAGGAGGU- -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 74401 | 0.68 | 0.99583 |
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Target: 5'- uGUUCaGGAAGGCc-UGCUcaCCUCCAa -3' miRNA: 3'- -UAAGaUCUUCCGcuAUGAaaGGAGGU- -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 158004 | 0.7 | 0.986735 |
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Target: 5'- uGUUCUGGAGGGCGGcg---UUCUCCc -3' miRNA: 3'- -UAAGAUCUUCCGCUaugaaAGGAGGu -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 48836 | 0.73 | 0.942386 |
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Target: 5'- --cCUGGGAGGUGAagaugGCUUcaaagUCCUCCAc -3' miRNA: 3'- uaaGAUCUUCCGCUa----UGAA-----AGGAGGU- -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 69980 | 0.78 | 0.754621 |
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Target: 5'- cAUUCUGGAAGGCGuagcguaugcguggACUggCCUCCAu -3' miRNA: 3'- -UAAGAUCUUCCGCua------------UGAaaGGAGGU- -5' |
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| 28892 | 3' | -48 | NC_006146.1 | + | 27546 | 1.08 | 0.019394 |
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Target: 5'- aAUUCUAGAAGGCGAUACUUUCCUCCAa -3' miRNA: 3'- -UAAGAUCUUCCGCUAUGAAAGGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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