Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28895 | 3' | -49.3 | NC_006146.1 | + | 9585 | 0.66 | 0.997622 |
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Target: 5'- uGCGCCUGCcccucACUGUUgGCAaaUGCAc -3' miRNA: 3'- cUGCGGAUGa----UGACGAgCGUaaAUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 168539 | 0.67 | 0.996646 |
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Target: 5'- gGACGCCUGucccgGCUCGUAcUUGCGc -3' miRNA: 3'- -CUGCGGAUgaugaCGAGCGUaAAUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 96266 | 0.67 | 0.995362 |
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Target: 5'- cGugGCCUACUACaggggGCgcCGCAUcaggcUGCAc -3' miRNA: 3'- -CugCGGAUGAUGa----CGa-GCGUAa----AUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 40097 | 0.68 | 0.992711 |
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Target: 5'- aGCGCgUGCUGCaGCUCGCuc--ACGc -3' miRNA: 3'- cUGCGgAUGAUGaCGAGCGuaaaUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 38104 | 0.7 | 0.968653 |
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Target: 5'- -cCGCCUGCaGCUGCUgaGCAgcUGCGa -3' miRNA: 3'- cuGCGGAUGaUGACGAg-CGUaaAUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 114554 | 0.71 | 0.949754 |
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Target: 5'- cACGCCUACUAcCUGCUC-CGg--GCGg -3' miRNA: 3'- cUGCGGAUGAU-GACGAGcGUaaaUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 133029 | 0.72 | 0.918628 |
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Target: 5'- -uCGCCgucuCUACUGCUCGCcgggUACGu -3' miRNA: 3'- cuGCGGau--GAUGACGAGCGuaa-AUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 124796 | 0.86 | 0.311769 |
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Target: 5'- cAUGCCUGCUGCUGCUCGCGcg-ACGg -3' miRNA: 3'- cUGCGGAUGAUGACGAGCGUaaaUGU- -5' |
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| 28895 | 3' | -49.3 | NC_006146.1 | + | 26411 | 1.1 | 0.011047 |
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Target: 5'- aGACGCCUACUACUGCUCGCAUUUACAa -3' miRNA: 3'- -CUGCGGAUGAUGACGAGCGUAAAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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