Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2890 | 5' | -56.5 | NC_001493.1 | + | 87579 | 0.74 | 0.427528 |
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Target: 5'- gGCGCUCauggguuCCGGGGGGgacacuacCGGAAGAGACc -3' miRNA: 3'- -UGUGAG-------GGCCCCCCac------GUCUUCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 9372 | 0.75 | 0.384018 |
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Target: 5'- gACACUCCuCGGGGGGUaGCuccagguacacgaAGAcGGGACg -3' miRNA: 3'- -UGUGAGG-GCCCCCCA-CG-------------UCUuCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 124926 | 0.75 | 0.384018 |
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Target: 5'- gACACUCCuCGGGGGGUaGCuccagguacacgaAGAcGGGACg -3' miRNA: 3'- -UGUGAGG-GCCCCCCA-CG-------------UCUuCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 65713 | 0.75 | 0.360164 |
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Target: 5'- --uCUCUCGGGcGGGUGUggccAGAAGAAGCa -3' miRNA: 3'- uguGAGGGCCC-CCCACG----UCUUCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 124696 | 0.77 | 0.292898 |
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Target: 5'- cGCGCUCCCGGGGauGGcgGCgagGGggGAGGCc -3' miRNA: 3'- -UGUGAGGGCCCC--CCa-CG---UCuuCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 9141 | 0.77 | 0.292898 |
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Target: 5'- cGCGCUCCCGGGGauGGcgGCgagGGggGAGGCc -3' miRNA: 3'- -UGUGAGGGCCCC--CCa-CG---UCuuCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 14820 | 1.1 | 0.001789 |
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Target: 5'- gACACUCCCGGGGGGUGCAGAAGAAACa -3' miRNA: 3'- -UGUGAGGGCCCCCCACGUCUUCUUUG- -5' |
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| 2890 | 5' | -56.5 | NC_001493.1 | + | 130374 | 1.1 | 0.001789 |
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Target: 5'- gACACUCCCGGGGGGUGCAGAAGAAACa -3' miRNA: 3'- -UGUGAGGGCCCCCCACGUCUUCUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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