Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28909 | 3' | -50.4 | NC_006146.1 | + | 128174 | 0.66 | 0.993734 |
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Target: 5'- -gCACCGcCCGGAUCCGcggcUGCCa- -3' miRNA: 3'- cgGUGGUaGGUCUAGGUuaa-ACGGau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 110488 | 0.66 | 0.993734 |
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Target: 5'- uCCACCccggCCAGGUCCAGgagggUGCa-- -3' miRNA: 3'- cGGUGGua--GGUCUAGGUUaa---ACGgau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 43701 | 0.66 | 0.994607 |
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Target: 5'- gGCCACUacggcGUCCAGGUCCuggcgGAUUU-CCUc -3' miRNA: 3'- -CGGUGG-----UAGGUCUAGG-----UUAAAcGGAu -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 53884 | 0.66 | 0.994607 |
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Target: 5'- cGCCcCCGUagggcguagcCCAGGUCCAGg--GCCc- -3' miRNA: 3'- -CGGuGGUA----------GGUCUAGGUUaaaCGGau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 95831 | 0.66 | 0.995379 |
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Target: 5'- gGCC-CCGgcUCCAGAUCCuguacugGCCc- -3' miRNA: 3'- -CGGuGGU--AGGUCUAGGuuaaa--CGGau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 95879 | 0.66 | 0.995379 |
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Target: 5'- gGCC-CCGguUCCAGAUCCuguacugGCCc- -3' miRNA: 3'- -CGGuGGU--AGGUCUAGGuuaaa--CGGau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 121472 | 0.66 | 0.996058 |
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Target: 5'- aUCGCCAacgCCGGGUCCAucGUggggGCCg- -3' miRNA: 3'- cGGUGGUa--GGUCUAGGU--UAaa--CGGau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 52467 | 0.66 | 0.996058 |
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Target: 5'- aGCCGCCGUgCAGGaCCucg--GCCa- -3' miRNA: 3'- -CGGUGGUAgGUCUaGGuuaaaCGGau -5' |
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| 28909 | 3' | -50.4 | NC_006146.1 | + | 120810 | 0.66 | 0.996058 |
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Target: 5'- cGCaACCGgagUCAGAUCCAgg-UGCCg- -3' miRNA: 3'- -CGgUGGUa--GGUCUAGGUuaaACGGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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