Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28917 | 3' | -53.6 | NC_006146.1 | + | 158247 | 0.67 | 0.93725 |
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Target: 5'- gGCCAcuggguCUUUAguuauGGGGCCUGGCCGg--- -3' miRNA: 3'- -UGGUu-----GAAGU-----CCUCGGACCGGUaacu -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 160073 | 0.67 | 0.941599 |
|
Target: 5'- gGCUGGCUUC-GGAGCCuaucucaUGGCCcaUGGg -3' miRNA: 3'- -UGGUUGAAGuCCUCGG-------ACCGGuaACU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 161319 | 0.69 | 0.889731 |
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Target: 5'- uGCCAACagUgAGGGGCa-GGCgCAUUGAa -3' miRNA: 3'- -UGGUUGa-AgUCCUCGgaCCG-GUAACU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 165807 | 0.72 | 0.723041 |
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Target: 5'- gGCCGGCUagGGGGGCguccCUGGCCGcgUGGa -3' miRNA: 3'- -UGGUUGAagUCCUCG----GACCGGUa-ACU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 167078 | 0.66 | 0.962527 |
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Target: 5'- cGCCGGCUcCaaaaaagugagGGGGGCgUGGCCAUc-- -3' miRNA: 3'- -UGGUUGAaG-----------UCCUCGgACCGGUAacu -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 167502 | 0.75 | 0.567321 |
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Target: 5'- uGCCAcuucacaggcacGCUUCAGG-GCCUguaggacgccgcGGCCGUUGAu -3' miRNA: 3'- -UGGU------------UGAAGUCCuCGGA------------CCGGUAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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