Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28917 | 3' | -53.6 | NC_006146.1 | + | 39800 | 0.68 | 0.909405 |
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Target: 5'- cCCGGCUUCuGGGAcGgC-GGCCGUUGAc -3' miRNA: 3'- uGGUUGAAG-UCCU-CgGaCCGGUAACU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 39406 | 0.72 | 0.750035 |
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Target: 5'- gGCCAcauggACUUgAagcagaaguuggccGGGGCCUGGCCcgUGAg -3' miRNA: 3'- -UGGU-----UGAAgU--------------CCUCGGACCGGuaACU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 31815 | 0.67 | 0.946643 |
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Target: 5'- cCCAACUcCAGGcgaGGCCcgUGGCCAa--- -3' miRNA: 3'- uGGUUGAaGUCC---UCGG--ACCGGUaacu -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 23348 | 0.67 | 0.94207 |
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Target: 5'- gACCGACcUCAGc-GCCUGGCgCGUgGAa -3' miRNA: 3'- -UGGUUGaAGUCcuCGGACCG-GUAaCU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 12691 | 1.08 | 0.004979 |
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Target: 5'- cACCAACUUCAGGAGCCUGGCCAUUGAc -3' miRNA: 3'- -UGGUUGAAGUCCUCGGACCGGUAACU- -5' |
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| 28917 | 3' | -53.6 | NC_006146.1 | + | 9929 | 0.69 | 0.870951 |
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Target: 5'- aGCCcGCgagCAGGAgcagcugagcgaaaaGCCUGGCCAUg-- -3' miRNA: 3'- -UGGuUGaa-GUCCU---------------CGGACCGGUAacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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