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miRNA: 3'- -CAGAG-GCUCuu--GGACga--CUGUCAGCG- -5'
miRNA: 3'- -CAGAGGCuCUUGGA--CGACUGUCAgcg -5'
miRNA: 3'- cAGAGGCUCUUGGa--CGACu--GUCagCG- -5'
miRNA: 3'- -CAGAGGcUCUUGGACGACUGUCAGCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 28933 | 3' | -53.9 | NC_006146.1 | + | 66275 | 0.71 | 0.810111 |
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Target: 5'- gGUCUCgCGGGuccuCCUGaucaGAUAGUCGCg -3' miRNA: 3'- -CAGAG-GCUCuu--GGACga--CUGUCAGCG- -5' |
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| 28933 | 3' | -53.9 | NC_006146.1 | + | 59280 | 0.71 | 0.801201 |
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Target: 5'- cGUCUUCGcGGACCUggGCUGGCAGUa-- -3' miRNA: 3'- -CAGAGGCuCUUGGA--CGACUGUCAgcg -5' |
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| 28933 | 3' | -53.9 | NC_006146.1 | + | 53729 | 0.71 | 0.792136 |
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Target: 5'- cUCUCCGGGAGCCgcgGCUGcgcCGGg-GCg -3' miRNA: 3'- cAGAGGCUCUUGGa--CGACu--GUCagCG- -5' |
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| 28933 | 3' | -53.9 | NC_006146.1 | + | 1990 | 1.01 | 0.017673 |
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Target: 5'- cGUCUCC-AGAACCUGCUGACAGUCGCg -3' miRNA: 3'- -CAGAGGcUCUUGGACGACUGUCAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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