Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28938 | 3' | -57.9 | NC_006146.1 | + | 90606 | 0.69 | 0.671855 |
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Target: 5'- gACCUCUGGUgGC-CGAG-GCAGUg- -3' miRNA: 3'- gUGGAGACCAgCGaGUUCgCGUCGgu -5' |
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| 28938 | 3' | -57.9 | NC_006146.1 | + | 43238 | 0.69 | 0.651623 |
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Target: 5'- gACCUUUGGggUGcCUCGgcgccAGCGCGGCCu -3' miRNA: 3'- gUGGAGACCa-GC-GAGU-----UCGCGUCGGu -5' |
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| 28938 | 3' | -57.9 | NC_006146.1 | + | 33202 | 0.69 | 0.64148 |
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Target: 5'- gGCCUCcgGGUgcuccuggUGCUCcGGgGCAGCCGg -3' miRNA: 3'- gUGGAGa-CCA--------GCGAGuUCgCGUCGGU- -5' |
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| 28938 | 3' | -57.9 | NC_006146.1 | + | 43331 | 0.7 | 0.59081 |
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Target: 5'- gGCCUC-GGcCGCccagggccagCAGGCGCAGCCc -3' miRNA: 3'- gUGGAGaCCaGCGa---------GUUCGCGUCGGu -5' |
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| 28938 | 3' | -57.9 | NC_006146.1 | + | 67151 | 0.74 | 0.402251 |
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Target: 5'- aGCCgCUGGcCGCUCcuGAGCGCGGCg- -3' miRNA: 3'- gUGGaGACCaGCGAG--UUCGCGUCGgu -5' |
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| 28938 | 3' | -57.9 | NC_006146.1 | + | 5736 | 1.07 | 0.002366 |
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Target: 5'- gCACCUCUGGUCGCUCAAGCGCAGCCAc -3' miRNA: 3'- -GUGGAGACCAGCGAGUUCGCGUCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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