miRNA: 3'- -UCACCCC------UCCCGUGU-----------GCGGu-------------CGACCG- -5'
miRNA: 3'- ucaCCCCUCCCG--------UGUGCGgUCg-ACCG- -5'
miRNA: 3'- -UCaCCCC-UCCCGugUGCGGUCgacCG- -5'
Results 141 - 143 of 143 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28944 | 3' | -61.9 | NC_006146.1 | + | 169595 | 0.67 | 0.628709 |
|
Target: 5'- gGGUGGGGccugagGGGGCGCGgcgauugucgcCGCCcucccucugcucccGUUGGCg -3' miRNA: 3'- -UCACCCC------UCCCGUGU-----------GCGGu-------------CGACCG- -5' |
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| 28944 | 3' | -61.9 | NC_006146.1 | + | 170059 | 0.7 | 0.436721 |
|
Target: 5'- --cGGGGAGGGCccggggccGCGCGUgGGgaUGGCc -3' miRNA: 3'- ucaCCCCUCCCG--------UGUGCGgUCg-ACCG- -5' |
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| 28944 | 3' | -61.9 | NC_006146.1 | + | 170304 | 0.7 | 0.402927 |
|
Target: 5'- gAG-GGGGcAGGGCugGCGCCGGgccGCg -3' miRNA: 3'- -UCaCCCC-UCCCGugUGCGGUCgacCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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