Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2899 | 5' | -57.3 | NC_001493.1 | + | 46336 | 0.67 | 0.81069 |
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Target: 5'- -cCCGGUCaucCCGCUCGacuCCACCa -3' miRNA: 3'- caGGCCAGcuaGGUGGGCguuGGUGG- -5' |
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| 2899 | 5' | -57.3 | NC_001493.1 | + | 62062 | 0.67 | 0.819278 |
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Target: 5'- -cCCGGUCG--UCGCCCGUugggauagcugAGCCGCg -3' miRNA: 3'- caGGCCAGCuaGGUGGGCG-----------UUGGUGg -5' |
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| 2899 | 5' | -57.3 | NC_001493.1 | + | 51982 | 0.67 | 0.827699 |
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Target: 5'- uUCCaGGUCGAgauucUCC-UCCGCGAaaaacucccCCGCCa -3' miRNA: 3'- cAGG-CCAGCU-----AGGuGGGCGUU---------GGUGG- -5' |
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| 2899 | 5' | -57.3 | NC_001493.1 | + | 9885 | 0.66 | 0.835947 |
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Target: 5'- -gCCGGUCGAgCCGCagcaGCAggaggggguACCGCUa -3' miRNA: 3'- caGGCCAGCUaGGUGgg--CGU---------UGGUGG- -5' |
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| 2899 | 5' | -57.3 | NC_001493.1 | + | 87620 | 0.65 | 0.871449 |
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Target: 5'- cGUCCGcgccgguagugcccGUCGAgucuucggaugacUCCGCCCGUggAGCCGgCu -3' miRNA: 3'- -CAGGC--------------CAGCU-------------AGGUGGGCG--UUGGUgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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