Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 2900 | 3' | -58.9 | NC_001493.1 | + | 27791 | 0.66 | 0.797012 |
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Target: 5'- -cCGGCCGGuGGUaucggGGCCGAUCccgAGCc -3' miRNA: 3'- gcGCUGGCC-CCA-----CCGGCUAGaugUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 32790 | 0.66 | 0.797012 |
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Target: 5'- cCGCGACCGGGaucGGCaCGcgCggaUAUGGCc -3' miRNA: 3'- -GCGCUGGCCCca-CCG-GCuaG---AUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 18811 | 0.66 | 0.797012 |
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Target: 5'- uGCGAgCGGGGggugagacucgGGUCGcUCgacGCGGCc -3' miRNA: 3'- gCGCUgGCCCCa----------CCGGCuAGa--UGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 62996 | 0.66 | 0.788123 |
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Target: 5'- aGCGACCGGcaccGUGaCCG-UCgcgACGGCg -3' miRNA: 3'- gCGCUGGCCc---CACcGGCuAGa--UGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 5296 | 0.66 | 0.760681 |
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Target: 5'- gGUGACCGGGG-GGCUG----GCGGg -3' miRNA: 3'- gCGCUGGCCCCaCCGGCuagaUGUCg -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 120850 | 0.66 | 0.760681 |
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Target: 5'- gGUGACCGGGG-GGCUG----GCGGg -3' miRNA: 3'- gCGCUGGCCCCaCCGGCuagaUGUCg -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 120898 | 0.66 | 0.769949 |
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Target: 5'- cCGUGACCGGaaacucucccuGGUcaCCGGUCcGCGGCu -3' miRNA: 3'- -GCGCUGGCC-----------CCAccGGCUAGaUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 5344 | 0.66 | 0.769949 |
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Target: 5'- cCGUGACCGGaaacucucccuGGUcaCCGGUCcGCGGCu -3' miRNA: 3'- -GCGCUGGCC-----------CCAccGGCUAGaUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 12838 | 0.66 | 0.805759 |
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Target: 5'- uCGCGuCCGGGGUcgGGgCGcUCgggauacucGCGGCg -3' miRNA: 3'- -GCGCuGGCCCCA--CCgGCuAGa--------UGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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