Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2900 | 3' | -58.9 | NC_001493.1 | + | 30937 | 0.71 | 0.496022 |
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Target: 5'- uGCGGCCGcGGUgacGGCgCGGUCgGCGGCc -3' miRNA: 3'- gCGCUGGCcCCA---CCG-GCUAGaUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 78408 | 0.71 | 0.496022 |
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Target: 5'- aCGCauuGugCGGGGUGuGCCGAUUUuuGGUu -3' miRNA: 3'- -GCG---CugGCCCCAC-CGGCUAGAugUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 32595 | 0.69 | 0.603345 |
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Target: 5'- uCGCGACCGGGacgGGC--GUUUACGGUc -3' miRNA: 3'- -GCGCUGGCCCca-CCGgcUAGAUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 48172 | 0.69 | 0.603345 |
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Target: 5'- uCGUGGCCGGGuugggGGCCGGguUCgACAGg -3' miRNA: 3'- -GCGCUGGCCCca---CCGGCU--AGaUGUCg -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 81414 | 0.68 | 0.643408 |
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Target: 5'- uGCGACCGcucaGGUGGuuGAUgUGCcGUg -3' miRNA: 3'- gCGCUGGCc---CCACCggCUAgAUGuCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 42789 | 0.67 | 0.716774 |
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Target: 5'- aGCGAUCGGGGUuaucggcgcuccuauGGCCc-UUUugAGCc -3' miRNA: 3'- gCGCUGGCCCCA---------------CCGGcuAGAugUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 5344 | 0.66 | 0.769949 |
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Target: 5'- cCGUGACCGGaaacucucccuGGUcaCCGGUCcGCGGCu -3' miRNA: 3'- -GCGCUGGCC-----------CCAccGGCUAGaUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 120898 | 0.66 | 0.769949 |
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Target: 5'- cCGUGACCGGaaacucucccuGGUcaCCGGUCcGCGGCu -3' miRNA: 3'- -GCGCUGGCC-----------CCAccGGCUAGaUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 43448 | 0.74 | 0.357959 |
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Target: 5'- uGUGGaucuCCGGGG-GGUCGAUCUgcGCGGCc -3' miRNA: 3'- gCGCU----GGCCCCaCCGGCUAGA--UGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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