Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2900 | 3' | -58.9 | NC_001493.1 | + | 3356 | 0.69 | 0.593362 |
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Target: 5'- aGCG-CCGGGGUGGUgaucgaCGAUgUACAc- -3' miRNA: 3'- gCGCuGGCCCCACCG------GCUAgAUGUcg -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 118910 | 0.69 | 0.593362 |
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Target: 5'- aGCG-CCGGGGUGGUgaucgaCGAUgUACAc- -3' miRNA: 3'- gCGCuGGCCCCACCG------GCUAgAUGUcg -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 64944 | 0.7 | 0.563605 |
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Target: 5'- gCGCGAuaaCCGGGGUGcGCCgcguGAUCguccagAgGGCg -3' miRNA: 3'- -GCGCU---GGCCCCAC-CGG----CUAGa-----UgUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 78408 | 0.71 | 0.496022 |
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Target: 5'- aCGCauuGugCGGGGUGuGCCGAUUUuuGGUu -3' miRNA: 3'- -GCG---CugGCCCCAC-CGGCUAGAugUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 30937 | 0.71 | 0.496022 |
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Target: 5'- uGCGGCCGcGGUgacGGCgCGGUCgGCGGCc -3' miRNA: 3'- gCGCUGGCcCCA---CCG-GCUAGaUGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 87269 | 0.71 | 0.486654 |
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Target: 5'- uCGCGACCGGGuUGGUgGAaaUGCGGa -3' miRNA: 3'- -GCGCUGGCCCcACCGgCUagAUGUCg -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 43448 | 0.74 | 0.357959 |
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Target: 5'- uGUGGaucuCCGGGG-GGUCGAUCUgcGCGGCc -3' miRNA: 3'- gCGCU----GGCCCCaCCGGCUAGA--UGUCG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 48624 | 0.76 | 0.267241 |
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Target: 5'- gGCGAUCGuGGGUGGCaCGGUCUugGuGUu -3' miRNA: 3'- gCGCUGGC-CCCACCG-GCUAGAugU-CG- -5' |
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| 2900 | 3' | -58.9 | NC_001493.1 | + | 28366 | 1.12 | 0.001017 |
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Target: 5'- uCGCGACCGGGGUGGCCGAUCUACAGCg -3' miRNA: 3'- -GCGCUGGCCCCACCGGCUAGAUGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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