Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29009 | 5' | -55.9 | NC_006146.1 | + | 16015 | 0.69 | 0.813149 |
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Target: 5'- gGCccuGGCCCCGcCCGGUggCCUUGGg -3' miRNA: 3'- gCGuc-CUGGGGCaGGUCAa-GGAAUCa -5' |
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| 29009 | 5' | -55.9 | NC_006146.1 | + | 12938 | 0.69 | 0.813149 |
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Target: 5'- gGCccuGGCCCCGcCCGGUggCCUUGGg -3' miRNA: 3'- gCGuc-CUGGGGCaGGUCAa-GGAAUCa -5' |
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| 29009 | 5' | -55.9 | NC_006146.1 | + | 28327 | 0.69 | 0.813149 |
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Target: 5'- gGCccuGGCCCCGcCCGGUggCCUUGGg -3' miRNA: 3'- gCGuc-CUGGGGCaGGUCAa-GGAAUCa -5' |
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| 29009 | 5' | -55.9 | NC_006146.1 | + | 19093 | 0.69 | 0.813149 |
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Target: 5'- gGCccuGGCCCCGcCCGGUggCCUUGGg -3' miRNA: 3'- gCGuc-CUGGGGCaGGUCAa-GGAAUCa -5' |
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| 29009 | 5' | -55.9 | NC_006146.1 | + | 111906 | 0.74 | 0.519353 |
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Target: 5'- aGCAGGGCUUCGUUCAGggacgcgUUCUUGGUg -3' miRNA: 3'- gCGUCCUGGGGCAGGUCa------AGGAAUCA- -5' |
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| 29009 | 5' | -55.9 | NC_006146.1 | + | 158126 | 0.76 | 0.435273 |
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Target: 5'- uGCAGGGCCgCGUCCAGgUCCggcAGg -3' miRNA: 3'- gCGUCCUGGgGCAGGUCaAGGaa-UCa -5' |
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| 29009 | 5' | -55.9 | NC_006146.1 | + | 38548 | 1.08 | 0.003644 |
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Target: 5'- aCGCAGGACCCCGUCCAGUUCCUUAGUg -3' miRNA: 3'- -GCGUCCUGGGGCAGGUCAAGGAAUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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