Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29029 | 3' | -53.1 | NC_006146.1 | + | 115921 | 0.69 | 0.914466 |
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Target: 5'- cGgCCACCccggGUAcgggggcagcGGUAgcgGCGGCGGCg -3' miRNA: 3'- aCaGGUGGaa--CAU----------CCAUa--CGCCGUCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 149035 | 0.7 | 0.874335 |
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Target: 5'- -uUCC-CCUUGUGGGg--GUGGCucGGCu -3' miRNA: 3'- acAGGuGGAACAUCCauaCGCCG--UCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 4666 | 0.7 | 0.85911 |
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Target: 5'- cGUCCuggGCCggGUGGGcgagGCGGgGGCa -3' miRNA: 3'- aCAGG---UGGaaCAUCCaua-CGCCgUCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 109802 | 0.7 | 0.85911 |
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Target: 5'- uUGUCUGCUUgaugcuagucaUGUAGGUGaGCgGGCAGUc -3' miRNA: 3'- -ACAGGUGGA-----------ACAUCCAUaCG-CCGUCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 64168 | 0.71 | 0.808555 |
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Target: 5'- aGUCUGCCagGcUGGGUG-GgGGCAGCa -3' miRNA: 3'- aCAGGUGGaaC-AUCCAUaCgCCGUCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 56568 | 0.73 | 0.72218 |
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Target: 5'- cGUCCACCUccGUcAGGg--GCGGCcgGGCg -3' miRNA: 3'- aCAGGUGGAa-CA-UCCauaCGCCG--UCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 128145 | 0.74 | 0.691591 |
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Target: 5'- -aUCCAaCUcGUAGGccgcguUGUGCGGCAGCa -3' miRNA: 3'- acAGGUgGAaCAUCC------AUACGCCGUCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 119146 | 0.75 | 0.598023 |
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Target: 5'- aGgCCACCUuuuacgcgUGUcGGGcUAUGCGGCGGCu -3' miRNA: 3'- aCaGGUGGA--------ACA-UCC-AUACGCCGUCG- -5' |
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| 29029 | 3' | -53.1 | NC_006146.1 | + | 66477 | 1.11 | 0.004042 |
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Target: 5'- cUGUCCACCUUGUAGGUAUGCGGCAGCc -3' miRNA: 3'- -ACAGGUGGAACAUCCAUACGCCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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