Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2903 | 3' | -56.5 | NC_001493.1 | + | 28422 | 0.71 | 0.586759 |
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Target: 5'- cGAGGCGgcGCUCCGCGA---GCAGa- -3' miRNA: 3'- -CUUCGCuuCGAGGCGCUgcuCGUCgu -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 62676 | 0.71 | 0.576465 |
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Target: 5'- -cGGCGAAGC-CCGCGG-GAGCcaacGGCAc -3' miRNA: 3'- cuUCGCUUCGaGGCGCUgCUCG----UCGU- -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 62718 | 0.71 | 0.576465 |
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Target: 5'- -cGGCGAAGC-CCGCGG-GAGCcaacGGCAc -3' miRNA: 3'- cuUCGCUUCGaGGCGCUgCUCG----UCGU- -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 62760 | 0.71 | 0.576465 |
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Target: 5'- -cGGCGAAGC-CCGCGG-GAGCcaacGGCAc -3' miRNA: 3'- cuUCGCUUCGaGGCGCUgCUCG----UCGU- -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 62802 | 0.71 | 0.576465 |
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Target: 5'- -cGGCGAAGC-CCGCGG-GAGCcaacGGCAc -3' miRNA: 3'- cuUCGCUUCGaGGCGCUgCUCG----UCGU- -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 63303 | 0.71 | 0.545854 |
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Target: 5'- -cGGCGAAGC-CCGCGggaGCGAaCGGCAc -3' miRNA: 3'- cuUCGCUUCGaGGCGC---UGCUcGUCGU- -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 95114 | 0.71 | 0.54181 |
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Target: 5'- cGGGCGGAGCUCCGUGucauuuaacagccCGAGgGGCu -3' miRNA: 3'- cUUCGCUUCGAGGCGCu------------GCUCgUCGu -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 94966 | 0.72 | 0.525737 |
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Target: 5'- ---aCGGAGCUCCGCccggggaucuACGAGCGGCGg -3' miRNA: 3'- cuucGCUUCGAGGCGc---------UGCUCGUCGU- -5' |
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| 2903 | 3' | -56.5 | NC_001493.1 | + | 29809 | 1.08 | 0.002218 |
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Target: 5'- cGAAGCGAAGCUCCGCGACGAGCAGCAc -3' miRNA: 3'- -CUUCGCUUCGAGGCGCUGCUCGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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