Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29033 | 3' | -52 | NC_006146.1 | + | 54038 | 0.69 | 0.946776 |
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Target: 5'- gCC-UGGCcggGCCGGAUGugGuggaggacgagaGCGUGGGu -3' miRNA: 3'- -GGaACCG---UGGCCUACugU------------UGUACCUu -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 51498 | 0.77 | 0.544004 |
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Target: 5'- ---aGGUACCGGAUGAUcaggcgaAACAUGGAAg -3' miRNA: 3'- ggaaCCGUGGCCUACUG-------UUGUACCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 46891 | 0.66 | 0.981995 |
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Target: 5'- uCCUgGGCACCuacaacGGGUGACGGagaagGGAGa -3' miRNA: 3'- -GGAaCCGUGG------CCUACUGUUgua--CCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 38959 | 0.67 | 0.980722 |
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Target: 5'- gCCUUGGCAuCCGGGUGggcccccagacucucGCAGag-GGGg -3' miRNA: 3'- -GGAACCGU-GGCCUAC---------------UGUUguaCCUu -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 33346 | 0.7 | 0.896465 |
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Target: 5'- uCCggGGCAgCCGGGUGGCcGCcgGUGGGu -3' miRNA: 3'- -GGaaCCGU-GGCCUACUGuUG--UACCUu -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 33224 | 0.66 | 0.987435 |
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Target: 5'- uCCggGGCAgCCGGGUGGCcGCcgGcGGGu -3' miRNA: 3'- -GGaaCCGU-GGCCUACUGuUGuaC-CUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 13831 | 0.7 | 0.889632 |
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Target: 5'- uUCUcGGUGCUGGcgGACAGCGuccUGGAAa -3' miRNA: 3'- -GGAaCCGUGGCCuaCUGUUGU---ACCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 4505 | 0.67 | 0.974952 |
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Target: 5'- --aUGGCACUGGcgGGcCAGCggGGGGu -3' miRNA: 3'- ggaACCGUGGCCuaCU-GUUGuaCCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 141 | 0.68 | 0.95907 |
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Target: 5'- --aUGGCGCCuGGAUGGCugccCGUGGu- -3' miRNA: 3'- ggaACCGUGG-CCUACUGuu--GUACCuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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