Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29033 | 3' | -52 | NC_006146.1 | + | 113295 | 0.67 | 0.972209 |
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Target: 5'- aCCUUGGCccccACCGG--GACAAaaGUGGGAa -3' miRNA: 3'- -GGAACCG----UGGCCuaCUGUUg-UACCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 70498 | 0.68 | 0.965757 |
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Target: 5'- gCCUucUGGCAaggGGAUGGCugaaaugAGCGUGGAGg -3' miRNA: 3'- -GGA--ACCGUgg-CCUACUG-------UUGUACCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 161727 | 0.68 | 0.962693 |
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Target: 5'- gCUUGGgGgUGGGUGGCAcgGUGGGAg -3' miRNA: 3'- gGAACCgUgGCCUACUGUugUACCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 141 | 0.68 | 0.95907 |
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Target: 5'- --aUGGCGCCuGGAUGGCugccCGUGGu- -3' miRNA: 3'- ggaACCGUGG-CCUACUGuu--GUACCuu -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 87360 | 0.68 | 0.95907 |
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Target: 5'- --aUGGgACUGGAUGACAAgcUGUGGGc -3' miRNA: 3'- ggaACCgUGGCCUACUGUU--GUACCUu -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 33346 | 0.7 | 0.896465 |
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Target: 5'- uCCggGGCAgCCGGGUGGCcGCcgGUGGGu -3' miRNA: 3'- -GGaaCCGU-GGCCUACUGuUG--UACCUu -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 65064 | 0.74 | 0.711837 |
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Target: 5'- cUCUUGGCAgcgccuCCGGAUGuCGGCcgGGGAc -3' miRNA: 3'- -GGAACCGU------GGCCUACuGUUGuaCCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 73440 | 1.09 | 0.00743 |
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Target: 5'- cCCUUGGCACCGGAUGACAACAUGGAAg -3' miRNA: 3'- -GGAACCGUGGCCUACUGUUGUACCUU- -5' |
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| 29033 | 3' | -52 | NC_006146.1 | + | 64852 | 0.66 | 0.988932 |
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Target: 5'- aCCUUcugccaGGCuccaaguCCGGAUGGgcggccacggcCAGCAUGGAc -3' miRNA: 3'- -GGAA------CCGu------GGCCUACU-----------GUUGUACCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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