Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29034 | 3' | -55.2 | NC_006146.1 | + | 114301 | 0.68 | 0.83616 |
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Target: 5'- cGCCggCGaggaGACCGGcgGCGGGCucGGCGc -3' miRNA: 3'- -UGGaaGU----UUGGCCa-CGCCCGu-UCGUu -5' |
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| 29034 | 3' | -55.2 | NC_006146.1 | + | 2476 | 0.68 | 0.83616 |
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Target: 5'- gGCggUguGGCCGGcGgGGGCAGGCAGc -3' miRNA: 3'- -UGgaAguUUGGCCaCgCCCGUUCGUU- -5' |
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| 29034 | 3' | -55.2 | NC_006146.1 | + | 128441 | 0.69 | 0.791934 |
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Target: 5'- gGCCg-CAGACCGG-GCGGGaggggGAGCGGg -3' miRNA: 3'- -UGGaaGUUUGGCCaCGCCCg----UUCGUU- -5' |
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| 29034 | 3' | -55.2 | NC_006146.1 | + | 90070 | 0.7 | 0.734049 |
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Target: 5'- aGCCUagGAGgCGGUGCagGGGgGAGCAGu -3' miRNA: 3'- -UGGAagUUUgGCCACG--CCCgUUCGUU- -5' |
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| 29034 | 3' | -55.2 | NC_006146.1 | + | 39998 | 0.75 | 0.450658 |
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Target: 5'- gGCCcgUAGugGCCGGUGUGGGCGuAGCAGg -3' miRNA: 3'- -UGGaaGUU--UGGCCACGCCCGU-UCGUU- -5' |
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| 29034 | 3' | -55.2 | NC_006146.1 | + | 74164 | 1.05 | 0.005115 |
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Target: 5'- cACCUUCAAACCGGUGCGGGCAAGCAAu -3' miRNA: 3'- -UGGAAGUUUGGCCACGCCCGUUCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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