Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29034 | 5' | -53.8 | NC_006146.1 | + | 85508 | 0.66 | 0.94736 |
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Target: 5'- -aUGGCUcuuGACCACACGCCcccuUGUGg- -3' miRNA: 3'- acACCGA---UUGGUGUGUGGcc--ACACaa -5' |
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| 29034 | 5' | -53.8 | NC_006146.1 | + | 58210 | 0.68 | 0.910434 |
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Target: 5'- aUGUGGUUGuucaguAgCGCACACCGGgggcGUGg- -3' miRNA: 3'- -ACACCGAU------UgGUGUGUGGCCa---CACaa -5' |
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| 29034 | 5' | -53.8 | NC_006146.1 | + | 164520 | 0.69 | 0.853655 |
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Target: 5'- cGUGGCUuuCCAcCGCcCCGGUGcUGa- -3' miRNA: 3'- aCACCGAuuGGU-GUGuGGCCAC-ACaa -5' |
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| 29034 | 5' | -53.8 | NC_006146.1 | + | 153430 | 0.71 | 0.792966 |
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Target: 5'- gUGUGGCUGGCUuuggAgGgGCCGGUGuUGUg -3' miRNA: 3'- -ACACCGAUUGG----UgUgUGGCCAC-ACAa -5' |
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| 29034 | 5' | -53.8 | NC_006146.1 | + | 96692 | 0.74 | 0.59004 |
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Target: 5'- ---aGCUcACCaACACACCGGUGUGUg -3' miRNA: 3'- acacCGAuUGG-UGUGUGGCCACACAa -5' |
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| 29034 | 5' | -53.8 | NC_006146.1 | + | 74201 | 1.06 | 0.006673 |
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Target: 5'- gUGUGGCUAACCACACACCGGUGUGUUg -3' miRNA: 3'- -ACACCGAUUGGUGUGUGGCCACACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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