Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2904 | 5' | -57 | NC_001493.1 | + | 92986 | 0.66 | 0.814994 |
Target: 5'- gGGUUCGGGGacgccGUCGgGUgggGCG-AGCUCg -3' miRNA: 3'- -UCGAGCUCU-----CAGCgCAa--CGCcUCGAG- -5' |
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2904 | 5' | -57 | NC_001493.1 | + | 8877 | 0.67 | 0.806165 |
Target: 5'- aAGCUCugauGGAG-CGCGUgGCGGGGUcCg -3' miRNA: 3'- -UCGAGc---UCUCaGCGCAaCGCCUCGaG- -5' |
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2904 | 5' | -57 | NC_001493.1 | + | 124431 | 0.67 | 0.806165 |
Target: 5'- aAGCUCugauGGAG-CGCGUgGCGGGGUcCg -3' miRNA: 3'- -UCGAGc---UCUCaGCGCAaCGCCUCGaG- -5' |
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2904 | 5' | -57 | NC_001493.1 | + | 82129 | 0.71 | 0.547015 |
Target: 5'- aGGCUCGAG-GUCuuccagGUGGAGCUCa -3' miRNA: 3'- -UCGAGCUCuCAGcgcaa-CGCCUCGAG- -5' |
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2904 | 5' | -57 | NC_001493.1 | + | 15121 | 0.74 | 0.396975 |
Target: 5'- cGGUUCGGGAGUCuCGggGCgcucaaGGAGCUCc -3' miRNA: 3'- -UCGAGCUCUCAGcGCaaCG------CCUCGAG- -5' |
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2904 | 5' | -57 | NC_001493.1 | + | 130676 | 0.74 | 0.396975 |
Target: 5'- cGGUUCGGGAGUCuCGggGCgcucaaGGAGCUCc -3' miRNA: 3'- -UCGAGCUCUCAGcGCaaCG------CCUCGAG- -5' |
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2904 | 5' | -57 | NC_001493.1 | + | 29862 | 1.08 | 0.002082 |
Target: 5'- cAGCUCGAGAGUCGCGUUGCGGAGCUCc -3' miRNA: 3'- -UCGAGCUCUCAGCGCAACGCCUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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