Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29051 | 3' | -50.8 | NC_006146.1 | + | 43355 | 0.7 | 0.944609 |
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Target: 5'- gGCGCAGCccguggcaGGggGUggcuuagcggGGGCAGCAg -3' miRNA: 3'- gCGUGUCG--------UCuuCAaaca------CCCGUUGU- -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 81606 | 0.71 | 0.914544 |
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Target: 5'- gGgACAagAGAAGgaUGUGGGCAGCGa -3' miRNA: 3'- gCgUGUcgUCUUCaaACACCCGUUGU- -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 150595 | 0.71 | 0.908322 |
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Target: 5'- aGCACccgccuGCAGggGa-UGUGGGCGAUc -3' miRNA: 3'- gCGUGu-----CGUCuuCaaACACCCGUUGu -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 100710 | 0.72 | 0.87344 |
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Target: 5'- gGCGCuGCGGggGUgggUGUGGGacCGGCc -3' miRNA: 3'- gCGUGuCGUCuuCAa--ACACCC--GUUGu -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 89462 | 0.72 | 0.86574 |
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Target: 5'- gGUGCAGCGGGAGUUUucUGGGUuGCGg -3' miRNA: 3'- gCGUGUCGUCUUCAAAc-ACCCGuUGU- -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 30121 | 0.73 | 0.838746 |
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Target: 5'- gGCACAcagagcgcccccauGCAGggGg--GUGGGCAugGc -3' miRNA: 3'- gCGUGU--------------CGUCuuCaaaCACCCGUugU- -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 42970 | 0.94 | 0.077244 |
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Target: 5'- aCGCugAGCGcGGAGUUUGUGGGCAGCAg -3' miRNA: 3'- -GCGugUCGU-CUUCAAACACCCGUUGU- -5' |
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| 29051 | 3' | -50.8 | NC_006146.1 | + | 92359 | 1.1 | 0.007875 |
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Target: 5'- gCGCACAGCAGAAGUUUGUGGGCAACAa -3' miRNA: 3'- -GCGUGUCGUCUUCAAACACCCGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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