Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29051 | 5' | -52.1 | NC_006146.1 | + | 48733 | 0.66 | 0.989561 |
Target: 5'- --gCUCGCgCAGCAGCUccuucucCUUGCGCc- -3' miRNA: 3'- cagGAGCG-GUUGUUGAa------GAACGCGcu -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 72997 | 0.66 | 0.988132 |
Target: 5'- --gCUCGCCu-CAGCUcCUUGUGCa- -3' miRNA: 3'- cagGAGCGGuuGUUGAaGAACGCGcu -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 127348 | 0.67 | 0.976161 |
Target: 5'- -aCCgcgUGCCGcgucuccuACuGCUUCUUGCGCGc -3' miRNA: 3'- caGGa--GCGGU--------UGuUGAAGAACGCGCu -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 42236 | 0.68 | 0.960829 |
Target: 5'- cUUUUUGCCAACGACccccUCcgGCGCGAg -3' miRNA: 3'- cAGGAGCGGUUGUUGa---AGaaCGCGCU- -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 46759 | 0.68 | 0.957858 |
Target: 5'- gGUCCUCGCCucccgaccccgggcgGAUGGCUggccggccgGCGCGAg -3' miRNA: 3'- -CAGGAGCGG---------------UUGUUGAagaa-----CGCGCU- -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 167027 | 0.71 | 0.886183 |
Target: 5'- -gCCUCGCCGagaccaGCAGCUUCUcGUucauGCGAu -3' miRNA: 3'- caGGAGCGGU------UGUUGAAGAaCG----CGCU- -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 117970 | 0.72 | 0.822677 |
Target: 5'- aUCCUCGCCAugAAaUUCUcaaaggUGCGCa- -3' miRNA: 3'- cAGGAGCGGUugUUgAAGA------ACGCGcu -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 67695 | 0.74 | 0.737668 |
Target: 5'- aGUCC-CGCC-GCAGCUUUUcgcGCGCGAa -3' miRNA: 3'- -CAGGaGCGGuUGUUGAAGAa--CGCGCU- -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 150182 | 0.77 | 0.560284 |
Target: 5'- gGUCCUcgcaggcCGCCAGCAGCUUCUuaauggccuUGCGCu- -3' miRNA: 3'- -CAGGA-------GCGGUUGUUGAAGA---------ACGCGcu -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 15902 | 0.82 | 0.340014 |
Target: 5'- -gCCUCgaGCCAGCAACUUCUgugccgggcccUGCGCGAg -3' miRNA: 3'- caGGAG--CGGUUGUUGAAGA-----------ACGCGCU- -5' |
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29051 | 5' | -52.1 | NC_006146.1 | + | 92393 | 1.09 | 0.006812 |
Target: 5'- uGUCCUCGCCAACAACUUCUUGCGCGAg -3' miRNA: 3'- -CAGGAGCGGUUGUUGAAGAACGCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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