Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29058 | 3' | -50.3 | NC_006146.1 | + | 43632 | 0.66 | 0.997197 |
Target: 5'- aGUGggCGGCG-GGGCGUGGccgccugGgGAGAGg -3' miRNA: 3'- -UACaaGUCGUaCUUGCACC-------UgCUCUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 155803 | 0.66 | 0.996739 |
Target: 5'- aGUGUgu-GCcUGggUGUGGugGAGuGg -3' miRNA: 3'- -UACAaguCGuACuuGCACCugCUCuC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 152725 | 0.66 | 0.996739 |
Target: 5'- aGUGUgu-GCcUGggUGUGGugGAGuGg -3' miRNA: 3'- -UACAaguCGuACuuGCACCugCUCuC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 149648 | 0.66 | 0.996739 |
Target: 5'- aGUGUgu-GCcUGggUGUGGugGAGuGg -3' miRNA: 3'- -UACAaguCGuACuuGCACCugCUCuC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 146570 | 0.66 | 0.996739 |
Target: 5'- aGUGUgu-GCcUGggUGUGGugGAGuGg -3' miRNA: 3'- -UACAaguCGuACuuGCACCugCUCuC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 143492 | 0.66 | 0.996739 |
Target: 5'- aGUGUgu-GCcUGggUGUGGugGAGuGg -3' miRNA: 3'- -UACAaguCGuACuuGCACCugCUCuC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 12394 | 0.67 | 0.990673 |
Target: 5'- gAUGUUCAGggaCGUGGACGcggcggUGGACGccGAGg -3' miRNA: 3'- -UACAAGUC---GUACUUGC------ACCUGCu-CUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 95008 | 0.68 | 0.986237 |
Target: 5'- ----aCGGCGgucaGGAUGUGGACgGAGAGg -3' miRNA: 3'- uacaaGUCGUa---CUUGCACCUG-CUCUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 143173 | 0.68 | 0.984445 |
Target: 5'- cUGUagAGUAUGggUGUGGuuuuagugcacGCGGGAGu -3' miRNA: 3'- uACAagUCGUACuuGCACC-----------UGCUCUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 103595 | 0.69 | 0.97273 |
Target: 5'- ---cUCAGCGcGGACGUGGccCGGGAGc -3' miRNA: 3'- uacaAGUCGUaCUUGCACCu-GCUCUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 68816 | 0.69 | 0.97273 |
Target: 5'- cGUGagCGGCAUcGAGa-UGGACGAGAGc -3' miRNA: 3'- -UACaaGUCGUA-CUUgcACCUGCUCUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 58216 | 0.71 | 0.927129 |
Target: 5'- uUGUUCAGUAgcgcacaccggGGGCGUGGACaAGGGc -3' miRNA: 3'- uACAAGUCGUa----------CUUGCACCUGcUCUC- -5' |
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29058 | 3' | -50.3 | NC_006146.1 | + | 95595 | 1.06 | 0.014977 |
Target: 5'- cAUGUUCAGCAUGAACGUGGACGAGAGc -3' miRNA: 3'- -UACAAGUCGUACUUGCACCUGCUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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