Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
29070 | 5' | -51.6 | NC_006146.1 | + | 103842 | 0.69 | 0.955213 |
Target: 5'- ----gGCCcGucuGGCagaGGUGGUCUCCGu -3' miRNA: 3'- gaaaaUGGaCu--UCGg--CCACCAGAGGU- -5' |
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29070 | 5' | -51.6 | NC_006146.1 | + | 63648 | 0.69 | 0.955213 |
Target: 5'- ---gUGCCUGAAGgugaUGGUgcuGGUCUCCu -3' miRNA: 3'- gaaaAUGGACUUCg---GCCA---CCAGAGGu -5' |
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29070 | 5' | -51.6 | NC_006146.1 | + | 96899 | 0.69 | 0.937353 |
Target: 5'- ----aGCgUGAGGCCguGGUGGUCaguuUCCAg -3' miRNA: 3'- gaaaaUGgACUUCGG--CCACCAG----AGGU- -5' |
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29070 | 5' | -51.6 | NC_006146.1 | + | 11138 | 0.7 | 0.909399 |
Target: 5'- ---cUACCUGggGgCGGUGGUggUCAc -3' miRNA: 3'- gaaaAUGGACuuCgGCCACCAgaGGU- -5' |
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29070 | 5' | -51.6 | NC_006146.1 | + | 89455 | 0.72 | 0.835401 |
Target: 5'- ----gGCgUGAAGCUGGUGGUUguuugCCAc -3' miRNA: 3'- gaaaaUGgACUUCGGCCACCAGa----GGU- -5' |
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29070 | 5' | -51.6 | NC_006146.1 | + | 106643 | 0.66 | 0.991509 |
Target: 5'- ----cGCCUGcugcGGCCGGcUGGUCagggCCGu -3' miRNA: 3'- gaaaaUGGACu---UCGGCC-ACCAGa---GGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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