Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29074 | 3' | -51.6 | NC_006146.1 | + | 124184 | 0.67 | 0.982941 |
|
Target: 5'- -gAUUCGCAaGGGGGGCGcugaGCAgGCAg -3' miRNA: 3'- ugUAGGCGUaUCUCCCGUa---UGUgUGU- -5' |
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| 29074 | 3' | -51.6 | NC_006146.1 | + | 127141 | 0.68 | 0.967453 |
|
Target: 5'- uGC-UCCGCGUGGAGcuggacGGCGUcAUGCGCGa -3' miRNA: 3'- -UGuAGGCGUAUCUC------CCGUA-UGUGUGU- -5' |
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| 29074 | 3' | -51.6 | NC_006146.1 | + | 155542 | 0.67 | 0.978098 |
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Target: 5'- cGCGUCCG---AGAGGGCGUuggagccgggcuCGCGCGg -3' miRNA: 3'- -UGUAGGCguaUCUCCCGUAu-----------GUGUGU- -5' |
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| 29074 | 3' | -51.6 | NC_006146.1 | + | 159219 | 0.69 | 0.939176 |
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Target: 5'- aAUAUCUGCGUggauaccguGGGGGGCcagGCugGCAu -3' miRNA: 3'- -UGUAGGCGUA---------UCUCCCGua-UGugUGU- -5' |
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| 29074 | 3' | -51.6 | NC_006146.1 | + | 165220 | 0.67 | 0.982941 |
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Target: 5'- aGCAccCCGUGUAGGGGGCAaAgGCGgAg -3' miRNA: 3'- -UGUa-GGCGUAUCUCCCGUaUgUGUgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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