Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29075 | 3' | -62.6 | NC_006146.1 | + | 10851 | 0.71 | 0.360258 |
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Target: 5'- -uGUGCCGCUCCUCuGCGgcaucaccGUGGaGCCg- -3' miRNA: 3'- guCACGGCGAGGAG-CGU--------CACC-CGGga -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 83509 | 0.71 | 0.352683 |
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Target: 5'- uGGUGCCuCUCCUggCGCAGgguucugaucaGGGCCCa -3' miRNA: 3'- gUCACGGcGAGGA--GCGUCa----------CCCGGGa -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 100034 | 0.72 | 0.312374 |
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Target: 5'- gGGUccGCgGCUUCUCGCGGUcguguucccucacucGGGCCCg -3' miRNA: 3'- gUCA--CGgCGAGGAGCGUCA---------------CCCGGGa -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 42640 | 0.73 | 0.247003 |
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Target: 5'- ---cGCCGUgggCCUcaCGUAGUGGGCCCa -3' miRNA: 3'- gucaCGGCGa--GGA--GCGUCACCCGGGa -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 102784 | 0.66 | 0.600932 |
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Target: 5'- uGGcGCCGCUCC-CGCGGaccggcUGGccGCCCa -3' miRNA: 3'- gUCaCGGCGAGGaGCGUC------ACC--CGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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