Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 29075 | 3' | -62.6 | NC_006146.1 | + | 148133 | 0.71 | 0.345222 |
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Target: 5'- gAGUGCCGCaugggCUUCGUgcuGGUcaGGGCCCg -3' miRNA: 3'- gUCACGGCGa----GGAGCG---UCA--CCCGGGa -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 50534 | 0.71 | 0.352683 |
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Target: 5'- uCAGgaGCCGCUCCagGUcccgGGUGGGCaCCUg -3' miRNA: 3'- -GUCa-CGGCGAGGagCG----UCACCCG-GGA- -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 54415 | 0.69 | 0.433368 |
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Target: 5'- ---cGCCGCUCCUcCGCGGUggagaGGGUCUc -3' miRNA: 3'- gucaCGGCGAGGA-GCGUCA-----CCCGGGa -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 57160 | 0.69 | 0.468516 |
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Target: 5'- ---cGCCGCuuccacgccuUCCUCacccCAGUGGGCCCc -3' miRNA: 3'- gucaCGGCG----------AGGAGc---GUCACCCGGGa -5' |
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| 29075 | 3' | -62.6 | NC_006146.1 | + | 45976 | 0.66 | 0.64019 |
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Target: 5'- cCGGgGCaGCUCCUCGCcccgcGUGuaGGCCCg -3' miRNA: 3'- -GUCaCGgCGAGGAGCGu----CAC--CCGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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