Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 29078 | 5' | -44 | NC_006146.1 | + | 129251 | 0.66 | 1 |
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Target: 5'- -cAGGUgagGCGcGAGGGUCAGGgaUGUGg -3' miRNA: 3'- guUUCA---UGUaUUCUCAGUCCa-ACACa -5' |
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| 29078 | 5' | -44 | NC_006146.1 | + | 53819 | 0.66 | 1 |
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Target: 5'- gCGGGGgGCuggAAGAGUCGGGcgaagGUGUg -3' miRNA: 3'- -GUUUCaUGua-UUCUCAGUCCaa---CACA- -5' |
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| 29078 | 5' | -44 | NC_006146.1 | + | 70164 | 0.66 | 0.999999 |
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Target: 5'- --uAGaGCAUGGGGGUgAGGUgGUGg -3' miRNA: 3'- guuUCaUGUAUUCUCAgUCCAaCACa -5' |
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| 29078 | 5' | -44 | NC_006146.1 | + | 140006 | 0.67 | 0.999999 |
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Target: 5'- -cAAGUGgGUuccuaucgcGGGGGUCAGGUucuUGUGUu -3' miRNA: 3'- guUUCAUgUA---------UUCUCAGUCCA---ACACA- -5' |
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| 29078 | 5' | -44 | NC_006146.1 | + | 89458 | 0.68 | 0.99999 |
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Target: 5'- aCGAGGUGCAgcgGGAGUUuucuGGGUUGcggGUa -3' miRNA: 3'- -GUUUCAUGUau-UCUCAG----UCCAACa--CA- -5' |
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| 29078 | 5' | -44 | NC_006146.1 | + | 64857 | 0.8 | 0.913647 |
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Target: 5'- -cAGGUGCAgAAGGGUCGGGUcUGUGUu -3' miRNA: 3'- guUUCAUGUaUUCUCAGUCCA-ACACA- -5' |
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| 29078 | 5' | -44 | NC_006146.1 | + | 108010 | 1.1 | 0.042217 |
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Target: 5'- cCAAAGUACAUAAGAGUCAGGUUGUGUg -3' miRNA: 3'- -GUUUCAUGUAUUCUCAGUCCAACACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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