Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 29086 | 5' | -59.7 | NC_006146.1 | + | 57873 | 0.68 | 0.583039 |
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Target: 5'- ---gCCGaCGUCCugCCGGGCGUCUGCu- -3' miRNA: 3'- uauaGGC-GUAGG--GGUCCGCGGACGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 48052 | 0.69 | 0.552946 |
|
Target: 5'- -cGUCCaGCG-CCCCGGGCaGCgUGCGg -3' miRNA: 3'- uaUAGG-CGUaGGGGUCCG-CGgACGUa -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 98396 | 0.71 | 0.447775 |
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Target: 5'- -cGUCCGCuccUCCgCAGGCG-CUGCAg -3' miRNA: 3'- uaUAGGCGu--AGGgGUCCGCgGACGUa -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 139154 | 0.71 | 0.438753 |
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Target: 5'- --cUCC-CGUCCCCcggggGGGCGCCUGUg- -3' miRNA: 3'- uauAGGcGUAGGGG-----UCCGCGGACGua -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 79055 | 0.73 | 0.339256 |
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Target: 5'- -gGUCCGaGUCCgCAGGCGCCggacGCAUg -3' miRNA: 3'- uaUAGGCgUAGGgGUCCGCGGa---CGUA- -5' |
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| 29086 | 5' | -59.7 | NC_006146.1 | + | 114319 | 1.03 | 0.002842 |
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Target: 5'- cAUAUCCGCAUCCCCAGGCGCCUGCAUc -3' miRNA: 3'- -UAUAGGCGUAGGGGUCCGCGGACGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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